非小细胞肺癌患者外周血LUNX mRNA表达及临床意义

目的：通过检测非小细胞肺癌（NSCLC）患者化疗前后外周血中肺特异性X蛋白（LUNX）mRNA的表达,探讨其对NSCLC微转移的诊断价值。方法：采用逆转录-聚合酶链反应（RT-PCR）方法,对54例NSCLC患者化疗前后、非肺癌恶性肿瘤患者36例、肺良性疾病患者16例及健康志愿者15例的外周血进行LUNX mRNA检测。结果：54例NSCLC患者化疗前后外周血LUNX mRNA的阳性表达率分别是48.1%（26/54）和25.9%（14/54）,差异比较有统计学意义（P〈0.05）;非肺癌恶性肿瘤患者、肺良性疾病患者及健康志愿者外周血LUNX mRNA的阳性表达率均为0,与NSCLC组比较均有统计学意义（P〈0.01）;LUNX mRNA的阳性表达率在化疗前后均随TNM分期增加而升高（P〈0.05）,与患者年龄、性别、病理类型及分化程度无明显相关性（P〉0.05）;NSCLC外周血LUNX mRNA表达与化疗近期疗效无关（P〉0.05）。结论：LUNX mRNA是一种检测NSCLC外周血微转移较好的特异性标志物;化疗后NSCLC外周血LUNX mRNA的表达比化疗前显著降低,表明有效化疗可消灭NSCLC患者的微转移癌细胞。

Expression of LUNX mRNA in peripheral blood of non-small cell lung cancer patients and its clinical significance

Objective： To study the expression of LUNX mRNA in peripheral blood of patients with non small cell lung cancer（NSCLC） treated by chemotherapy,and evaluate its clinical value for diagnosis of micro-metastasis.By comparative analysis,it is to investigate the correlation among micro-metastasis,diagnosis,stage and the response of chemothe-rapy in NSCLC patients.Methods： RT-PCR was used to detect the LUNX mRNA in peripheral blood from 54 patients with NSCLC before and after platinum-based regimens in the combined chemotherapy,36 patients with malignant tumors except for lung cancer,16 patients with benign lung diseases and 15 healthy volunteers were served as controls.Results： The rate of positive expression of LUNX mRNA in peripheral blood of patients with NSCLC were 48.1%（26/54） and 25.9%（14/54）,respectively before and after the treatment（P〈0.05）.The rate of positive expression of LUNX mRNA were 0%（0/36）,0%（0/16） and 0%（0/15）in blood samples of 36 malignant tumors except lung cancer,16 benign lung diseases and 15 healthy volunteers respectively.There was significant different compare with NSCLC（P〈0.01）.The rate of positive expression of LUNX mRNA in peripheral blood of patients with NSCLC was significantly related to TNM stage（P〈0.05）.It had no obvious correlation with age,gender,pathological types or differentiation degree（P〈0.05）.The expression of LUNX mRNA in peripheral blood of patients with NSCLC had no obvious correlation with near future curative effect of chemotherapy.Conclusion： LUNX mRNA in peripheral blood can be used as prospective biomarkers for detecting micro-metastasis in patients with NSCLC.The rate of positive expression of LUNX mRNA in peripheral blood of patients with NSCLC was significantly related to TNM stage（P〈0.05）.There was not obvious correlation with age,gender,pathological types or differentiation degree（P〈0.05）.The rate of positive expression of LUNX mRNA in peripheral blood of patients with NSCLC was significantly descended after chemotherapy.Valid chemotherapy could eliminate the micro-metastasis cell in peripheral blood of patients with NSCLC.