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桩蛋白在大鼠星形胶质细胞中的表达
引用本文:张志军,何江虹,夏春林,倪衡建.桩蛋白在大鼠星形胶质细胞中的表达[J].南通医学院学报,2011,31(3):159-161,165,F0002.
作者姓名:张志军  何江虹  夏春林  倪衡建
作者单位:1. 南通大学杏林学院,南通,226001;南通大学医学院人体解剖学系,江苏省神经再生重点实验室;苏州大学医学部解剖学系暨细胞神经生物学研究室
2. 南通大学医学院人体解剖学系,江苏省神经再生重点实验室
3. 苏州大学医学部解剖学系暨细胞神经生物学研究室
4. 南通大学杏林学院,南通,226001;南通大学医学院人体解剖学系,江苏省神经再生重点实验室
基金项目:江苏高校优势学科建设工程资助项目;南通市科技项目,南通大学自然科学基金资助项目,南通大学杏林学院科研基金项目
摘    要:目的:初步探讨桩蛋白(paxillin)在大鼠星形胶质细胞及神经元中的表达。方法:通过组织切片、细胞培养、免疫荧光和RT-PCR方法,观察桩蛋白在大脑皮层及体外培养的星形胶质细胞以及神经元中的表达。结果:桩蛋白在大鼠脑皮质的星形胶质细胞中表达,神经元未见明显表达;在体外培养的星形胶质细胞以及神经元中的表达情况与体内一致,主要表达于星形胶质细胞的胞浆及突起中。结论:桩蛋白主要表达于星形胶质细胞而非神经元中。本实验结果为进一步研究桩蛋白在星形胶质细胞中的生物学作用奠定了基础。

关 键 词:桩蛋白  星形胶质细胞  神经元  反转录-聚合酶链式反应  免疫荧光染色  大鼠

Expression of paxillin in the rat astrocytes
ZHANG Zhijun,HE Jianghong,XIA Chunlin,NI Hengjian.Expression of paxillin in the rat astrocytes[J].ACTA Academiae Medicinae Nantong,2011,31(3):159-161,165,F0002.
Authors:ZHANG Zhijun  HE Jianghong  XIA Chunlin  NI Hengjian
Institution:1Xinglin College of Nantong University,Nantong 226001;2Department of Anatomy,Jiangsu Key Laboratory of Neuroregeneration,Medical College of Nantong University;3Department of Anatomy & Cytoneurobiology Unit,Medical College of Soochow University)
Abstract:Objective: To study the expression of paxillin in astrocytes and neurons of rat brain.Methods: The expression of paxillin was investigated in astrocytes and neurons by tissue section,cell culture,immunofluorescence and RT-PCR methods.Results: In the cortex,paxillin expressed mainly in astrocytes instead of the neurons.RT-PCR showed that paxillin mRNA was present in cultured astrocytes,but not in cultured neurons.Immunocytochemistry revealed that paxillin was located in the astrocytic cytoplasm and cell processes but not in the nucleus.Conclusion: Paxillin was expressed in astrocytes not in neurons.These findings contribute to a clearer understanding of paxillin in astrocytes.
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