The use of flow cytometry for the detection of CMV-specific antigen (pp65) in leukocytes of kidney recipients |
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| Authors: | Essa S Pacsa A S Al-Attiyah R El-Shazly A Raghupathy R Said T |
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| Institution: | Department of Microbiology, Faculty of Medicine, University of Kuwait. sahar@hsc.kuniv.edu.kw |
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| Abstract: | Flow cytometric assay (FCA) was used to detect a cytomegalovirus (CMV) specific antigen (pp65) in CMV-infected fibroblast cells and in leukocytes of kidney recipients. FCA distinguished clearly between the infected and non-infected fibroblast cells. Regarding transplant patients, the FCA was positive when the number of antigenemia assay (AA) positive cells was five or more per 5 x 10(4). Moreover, the percentage of antigenemia-positive cells by FCA correlated well with symptomatic CMV infections. |
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| Keywords: | CMV flow cytometry pp65 assay renal transplants |
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