抗rSjCTPI单克隆抗体检测血吸虫循环抗原疗效考核价值研究

目的 进一步探讨抗重组血吸虫磷酸丙糖异构酶（rSjCTPI）的单克隆抗体检测血吸虫循环抗原对血吸虫病疗效考核的价值。方法 建立以纯化的抗日本血吸虫可溶性虫卵抗原（SEA）的多克隆抗体（IgG）为捕捉系统,以酶标抗rSjCTPI为检测系统的酶联免疫吸附试验（P-M-ELISA）法,分别对同批治疗前及治疗后2、4、6、12个月的血吸虫病人进行检测,并与常规检测抗体的SEA-ELISA法比较。结果 P-M-ELISA法对治疗前血吸虫病人检测阳性率为96.6%,而检测抗体的SEA-ELISA为98.3%,P-M-ELISA法对治愈后2、4、6、12个月血清循环抗原检测阴转率分别为15.3%、37.3%、52.0%、83.3%,而检测抗体的SEA-ELISA法分别为3.4%、6.8%、12.0%、16.7%。结论 表明该法既有较好的诊断价值,又有较好的疗效考核价值。

STUDY ON EVALUATION OF EFFYCACY OF CHEMOTHERAPY ON DETECTION OF CIRCU-LATING SCHISTOSOME ANTIGEN WITH ANTI-rSjCTPI MONOCLONAL ANTIBODY

Objective To further explore the value on evaluation of chemotheraphy efficacy of schistosomiasis on detection of circulating antigen with anti-rSjCTPI monoclonal antibody. Methods P- M-ELISA was established with anti-SEA polyclonal antibody IgG to be as the capture antibody, and anti- rSjCTPI monoclonal antibody linked with HRP to be as detecting antibody. P- M-ELISA was applied for detection of circulating antigen in serum samples of patients with schistosomasis before and 2?4?6?12 months after treatment, and compared with SEA-ELISA for detecting of antibody. Results The positive rate was 96 6% of P- M-ELISA for detecting circulating antigen of patients with schistosomasis before treatment, with SEA-ELISA for detecting antibody was 98 3%. The negative conversion rates of patients at 2?4?6?12 months after treatment were 15 3%?37 3%?52 0%?83 3% respectively, but with SEA-ELISA were 3 4%?6 8%?12 0%?16 7%, respectively. Conclusion P- M-ELISA has high value on diagnosis and evaluation of chemotheraphy efficacy of schistosomiasis.