酶法测定血清钾离子试剂改进及评估

目的建立一种酶法测定血清钾离子液体试剂的方法，并评估该液体试剂的重复性（批内精密度）、抗干扰能力、线性、热稳定性，与离子选择电极法fISE）的相关性。方法对原有酶法测定血清钾离子方法进行了改进，并参考临床和实验室标准协会（CLSI）相关文件对试剂进行评估。结果批内精密度0.846％～1．338％；线性范围0.～10mnl01／L（γ=0.999）；干扰物浓度总胆红素（TBil）600mg／L，直接胆红素（DBil）600.mg／L，维生素C（Vc）60.0.mg／L，乳糜15mg／L，血红蛋白10g／L时对试剂干扰不明显（≤±10％），抗钠离子干扰为249；该液体试剂与ISE法的回归方程Y=1．0345X＋0.0753，相关系数1为0.984（P〈0．001），显著相关；试剂热稳定性在37℃下放置7d后初始吸光度值降低15．43％。结论试剂改良后线性、批内精密度、抗干扰能力、热稳定性、与ISE显著相关。

The improvement and assessment for method of enzymatic determination of potassium in serum

Objective ] hnproving the method of determination of potassium in serum which used to be freeze-dry powder powder.Testing the repeatability（within run precision）,interference,linearity, stability of the new method. Made the comparison test with ISE. Methods ] We Improved the enzymic method evaluated the new method according to the CLSI documents. Resluts ] Repeatability： CV between 0.846%-1.338%;the linearity range（ 0-10 retool/L）, γ =0.999;No significant interference of the new method was found when total bilirubin 600 mg/L, direct bilirubin 600 mg/L, vitamin C 600 mg/L,turbidity 15mg/L,hemoglobin 10 g/L; Compare with ISE method,Y= 1.0345 X＋0.0753, γ =0.984（P〈0.001）;Thermo-stability：the initial ABS decreased 15.43% compare with the control which is stored at 2 ℃ -8 ℃2 . Conclusion ] Compare with the freeze-dry powder reagent,the new reagent＇s linearity,repeatability,stability are acceptable,and significant correlation with ISE method.