PCR-SSCP银染法检测结核分枝杆菌rpsL基因突变

目的 探讨结核分枝杆菌对链霉素（SM）的耐药分子机制，建立快速、准确的SM耐药性检测方法。方法 应用PCR－SSCP银染分析技术，检测87例结核分枝杆菌临床分离株链霉素耐药性及rpsL基因变异情况，以结核分枝杆菌H37Rv标准株做对照。结果 25株对SM敏感的临床分离株rpsL基因未见异常，62株耐SM的临床分离析中48株rpsL基因SSCP分析结果异常。结论 rpsL基因突变是SM耐药性的重要分子机制，PCR－SSCP技术可以快速、准确地检测结核分枝杆菌对SM的耐药性。

Detection of Rpsl Gene Mutation of Mycobacterium Tuberculosis by PCR-SSCP Silver Staining

Objective:To study the value of detection of rpsl gene mutation in streptomy cin(SM)-resistance in clinical isolates of mycobacterium tuberculosis by PCR -SSCP,and to expect to set up a r apid detective method of rpsl gene mutation in mycobacterium tuberculosis,Method Eighty seven specimens isolated from patients were detected by PCR-SSCP silver staining,and using strain H 37Rv as a control,Results In 87 mycobacterium tuberculosis clinical isolates,the r psl DNA fragments from 16 drug-susceptible isolates had no mutation in rpsL gene ,48 of 62 sputum specimens showed rpsl gene mutation by PCR-SSCP,positive rate w as 77.4%.Conclusions The rpsL gene mutation is an important molecular mechanism of M.tuberc ulosis resistance to SM.PCR-SSCP might become a rapid detecting method of SM-r esistance of M.tuberculosis.