产肠毒素大肠杆菌(ETEC)的两种抗原制品对蛋鸡免疫原性的对比研究

目的：采用产肠毒素大肠杆菌（ETEC）K88标准菌株，研究其不同浓度的菌毛及全菌两种抗原对蛋鸡的免疫原性，并确定最佳的抗原浓度。方法：用SDS—PAGE鉴定菌毛蛋白的纯度，通过间接ELISA法检测特异性卵黄抗体（IgY）的效价。结果：菌毛蛋白对蛋鸡的免疫原性优于全菌；纯化的菌毛蛋白对蛋鸡的免疫原性优于粗菌毛蛋白，其诱导的抗体效价最高可达1：480000，初免84天后未见有明显的下降趋势；菌毛蛋白最佳浓度为2mg／ml，全菌最佳浓度为10^10cfu／ml。结论：本研究为制备高效价特异性IgY抗体奠定了基础。

The effect of immunogenicity of antigenic preparations from enterotoxigenic Escherichia coli on laying hens

Objective:Immunogenicity on laying hens was investigated following immunized with different antigen preparations. The antigen preparations used for the comparative studies were crude fimbriae, purified fimbriae and formalin-killed bacteria.Methods:The purity was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE). The antibody titer was measured by ELISA using purified fimbriae as an antigen.Results:The antibody titer was much greater when the fimbriae was used as an antigen(480 000) compared with that of antibodies with the formalin-killed bacteria(320 000). Moreover the purified fimbriae dramatically increased the titer. The optimal concentration of purified fimbriae and formalin-killed bacteria was found to be 2 mg/ml and 10 10 cfu/ml respectively, which exclited a maximal and stable antibody titer.Conclusion:The study can provide a novel alternative approach to obtain specific high titer IgY.