剥夺卵巢雌激素对成年大鼠基底前脑神经元表达Nestin的影响

目的 探讨剥夺卵巢雌激素对成年大鼠基底前脑神经元表达Ｎｅｓｔｉｎ的影响。方法 将健康成年雌性大鼠随机分为正常对照、卵巢切除２周及４周三组，用免疫组化法观察卵巢切除后基底前脑的内侧隔核（ＭＳ）、斜角带垂直支（ｖＤＢ）及水平支（ｂＤＢ）Ｎｅｓｔｉｎ免疫反应阳性（Ｎｅｓｔｉｎ－ＩＲ） 神经元的形态和数目变化。结果 卵巢切除２周、４周组ＭＳ、ｖＤＢ及ｈＤＢ的Ｎｅｓｈｎ－ＩＲ神经元形态与正常对照组相比无明显变化；但卵巢切除２周、４周组ＭＳ、ｖＤＢ及ｈＤＢ的 Ｎｅｓｔｉｎ－ＩＲ神经元的数目与正常对照组相比均有不同程度的减少，尤以ｈＤＢ的减少最明显（Ｐ＜０．０１，Ｐ＜０．０５）。结论 提示基底前脑Ｎｅｓｔｉｎ－ＩＲ神经元的Ｎｅｓｈｎ表达受卵巢雌激素的影响，ｈＤＢ的Ｎｅｓｈｎ－ＩＲ神经元的表达受此影响更大。

Effects of Ovarian Estrogen Deprivation on the Expression of Nestin in the Basal Forebrain Neurons of Adult Female Rats

Objective To investigate the effects of ovarian estrogen deprivation on the expression of nestin in the basal forebrain neurons of adult female rats. Methods Healthy adult female SD rats were randomly divided into three groups: ovary-intact, ovariectomized for two and four weeks. Immunohistochemical technigue was used to identify and compare the shapes and numbers of nestin-immunoreactive neurons in the medial septum (MS) , vertical (vDB) and horizontal (hDB) limbs of the diagonal band of Broca among the three groups. Results The shapes of nestin-immunoreactive neurons in MS, vDB and hDB of ovariectomized groups were similar to those of ovary-intact group. The numbers of nestin-immunoreactive neurons in both MS and vDB slightly decreased with a significant decrease in hDB of ovariectomized groups compared with those of ovary-intact group. Conclusion Deprivation of estrogen after ovariectomy produces a regionally selective effect on the expression of nestin in the basal forebrain neurons of adult female rats, reflecting that the expression of nestin in hDB neurons appears to be more dependent on the stimulation derived from ovarian estrogen.