| OD-PTD融合蛋白对K562细胞的作用 |
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| 引用本文： | 叶盛开,陈丽,刘宇鹏,刘丽娟. OD-PTD融合蛋白对K562细胞的作用[J]. 临床军医杂志, 2008, 36(4) |
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| 作者姓名： | 叶盛开 陈丽 刘宇鹏 刘丽娟 |
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| 作者单位： | 解放军第210医院,呼吸内科,辽宁,大连,116021;解放军第210医院,呼吸内科,辽宁,大连,116021;解放军第210医院,呼吸内科,辽宁,大连,116021;解放军第210医院,呼吸内科,辽宁,大连,116021 |
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| 摘 要： | 目的研究OD-PTD融合蛋白对K562细胞的作用。方法应用细胞计数的方法研究OD-PTD融合蛋白对K562细胞的生长曲线的影响;用MTT研究OD-PTD融合蛋白对K562细胞的细胞毒作用;用流式细胞仪技术研究OD-PTD融合蛋白对K562细胞周期的影响。结果OD-PTD融合蛋白能显著抑制细胞的生长(P<0.01);并且对K562细胞具有明显的细胞毒作用(P<0.01)。OD-PTD融合蛋白作用于K562细胞后,细胞周期变化明显,细胞从G0/G1期到S期发生明显阻滞(P<0.05)。结论OD-PTD融合蛋白对K562细胞有明显的抑制作用,为进一步探讨慢性粒细胞白血病新的治疗手段提供了实验依据。
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| 关 键 词： | 寡聚化结构域 K562细胞 细胞周期 增殖 |
Effects of OD-PTD Fusion Protein on K562 Cells |
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| Ye Sheng-kai,Chen Li,Liu Yu-peng,Liu L-juan. Effects of OD-PTD Fusion Protein on K562 Cells[J]. Clinical Journal of Medical Officer, 2008, 36(4) |
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| Authors: | Ye Sheng-kai Chen Li Liu Yu-peng Liu L-juan |
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| Affiliation: | Ye Sheng-kai,Chen Li,Liu Yu-peng,Liu L-juan (Department of Respiratory Medicine,PLA No.210 Hospital,Dalian 116021,China) |
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| Abstract: | Objective To explore the effects of OD-PTD fusion protein on K562 cells.Methods Proliferation ability of K562 cells treated by OD-PTD fusion protein was studied by growth curve. The cytotoxicity of OD-PTD fusion protein to K562 cells was determined by MTT assay. K562 cells' cycle change induced by OD-PTD fusion protein was investigated by flow cytometry. Results OD-PTD fusion protein significantly inhibited the growth of K562 cells, and it had a remarkablely cytotoxic effect on K562 cells. After several hou... |
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| Keywords: | oligomerization domain K562 cell cell cycle proliferation |
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