| 生长激素联合氟尿嘧啶对结肠癌LOVO细胞影响的实验研究 |
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| 引用本文： | 王琳,刘华,谢贤和,李苏宜,陈岩菊,赵英,白殿卿,麦泽锋.生长激素联合氟尿嘧啶对结肠癌LOVO细胞影响的实验研究[J].肠外与肠内营养,2009,16(6). |
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| 作者姓名： | 王琳 刘华 谢贤和 李苏宜 陈岩菊 赵英 白殿卿 麦泽锋 |
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| 作者单位： | 1. 海南省人民医院肿瘤内科,海南海口,570311
2. 东南大学附属中大医院临床肿瘤中心,江苏南京,210009 |
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| 摘 要： | 目的: 探讨生长激素(GH)联合氟尿嘧啶(5-Fu)在体外对结肠癌LOVO细胞的影响. 方法: 将培养的结肠癌LOVO细胞分为对照组、GH组、5-Fu组和GH+5-Fu组,GH组又按剂量分为两个亚组,即GH1终浓度为50 ng/mL和GH2终浓度为100 ng/mL;GH+5-Fu组又按剂量分为两个亚组,即GH1+5-Fu(GH 50 ng/mL+5-Fu 100 μg/mL)和GH2+5-Fu(GH 100 ng/mL+5-Fu 100 μg/mL),加入不同浓度的处理液后24、48和72 h,分别行四基偶氮唑蓝(MTT)比色观察,计算细胞存活率.并于加入不同浓度的处理液24 h后,以流式细胞仪测定细胞周期和凋亡情况.结果: 5-Fu组和GH+5-Fu组与对照组比较,细胞存活率明显下降(P＜0.05).GH+5-Fu组与单用5-Fu组比较,细胞生存率均有所降低,但各时间段无显著性差异(P＞0.05).GH组与对照组比较,S期细胞百分比和增殖指数(PI)均增加(P＜0.05),G_0/G_1细胞百分比有所减少(P＜0.05).GH+5-Fu组的S期细胞百分比和PI均降低(P＜0.05).5-Fu组和GH+5-Fu组的细胞凋亡率均升高(P＜0.05);GH+5-Fu组与5-Fu组比较,S期细胞百分比、PI和凋亡率均无显著性差异(P＞0.05). 结论: rhGH体外作用于结肠癌LOVO细胞,未刺激细胞生长,GH与化疗联合用是安全的.
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| 关 键 词： | 生长激素 5-氟尿嘧啶 LOVO细胞 |
Effects of recombinant human growth hormone and fluorouracil on human colon carcinoma LOVO cells in vitro |
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| WANG Lin,LIU Hua,XIE Xian-he,LI Su-yi,CHEN Yan-ju,ZHAO Ying,BAI Dian-qing,MAI Ze-feng.Effects of recombinant human growth hormone and fluorouracil on human colon carcinoma LOVO cells in vitro[J].Parenteral & Enteral Nutrition,2009,16(6). |
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| Authors: | WANG Lin LIU Hua XIE Xian-he LI Su-yi CHEN Yan-ju ZHAO Ying BAI Dian-qing MAI Ze-feng |
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| Institution: | WANG Lin1,LIU Hua1,XIE Xian-he1,LI Su-yi2,CHEN Yan-ju1,ZHAO Ying1,BAI Dian-qing1,MAI Ze-feng1 (1.Department of Oncology,People's Hospital of Hainan Province,Haikou 570311,Hainan,China,2.Clinical Cancer Center,Zhongda Hospital,Southeast University,Nanjing 210009,Jiangsu,China) |
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| Abstract: | Objective: To investigate the effects of recombinant human growth hormone(rhGH) and 5-fluorouracil(5-Fu) on human colon carcinoma LOVO cells in vitro.Methods: The LOVO cells during exponential growth stage were harvested and divided into control group,GH group,5-Fu group and GH+5-Fu group.According to the dose of GH,the GH group was separated into two sub-groups(50 ng/mL and 100 ng/mL) and the GH+5-Fu group was separated into two sub-groups.With different concentrations of rhGH and/or 5-Fu,the cell survive ... |
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| Keywords: | Growth hormone 5-fluorouracil LOVO cells |
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