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| Cloning of the Gene Encoding Urease Subunit.A in Helicobacter Pylori | |
| 引用本文： | 施理,张宜俊,陈劼,候晓华. Cloning of the Gene Encoding Urease Subunit.A in Helicobacter Pylori[J]. 华中科技大学学报(医学英德文版), 2004, 24(1): 22-24. DOI: 10.1007/BF02830697 |
| 作者姓名： | 施理 张宜俊 陈劼 候晓华 |
| 作者单位： | Infective Disease Center，Infective Disease Center，Guangzhou Chinese Traditional Medical University，Department of Digestive Disease Nanfang Hospital，Guangzhou 510602，Nanfang Hospital，Guangzhou 510602，Guangzhou 510405，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430022 |
| 摘 要： | Themostefficient ,economicalapproachwithlowriskofadversereactiontothepreventionandcon trolofHelicobacterpylori (H .pylori)infectionistousevaccine .UreaseanditssubunitAisproventobethemainvaccineagainsttheinfectionofH .pylori.Inordertoexpresstheureasesubuni… |
| 关 键 词： | 尿素酶亚型A 克隆 幽门螺杆菌 聚合酶链反应 基因工程 疫苗 免疫疗法 |
| 收稿时间： | 2002-02-15 |
Cloning of the gene encoding urease subunit a in helicobacter pylori |
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| Shi Li,Zhang Yijun,Chen Jie,Hou Xiaohua. Cloning of the gene encoding urease subunit a in helicobacter pylori[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2004, 24(1): 22-24. DOI: 10.1007/BF02830697 | |
| Authors: | Shi Li Zhang Yijun Chen Jie Hou Xiaohua |
| Affiliation: | 1. Infective Disease Center, Nanfang Hospital, Guangzhou 510602 2. Guangzhou Chinese Traditional Medical University, Guangzhou 510405 3. Department of Digestive Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 450022 |
| Abstract: | The gene encoding urease subunit A (ureA) of Helicobacter pylori (H.pylori) was cloned from H.pylori isolate by polymerase chain reaction (PCR). Sterile distilled water instead of DNA served as negative control. The nucleotide sequence of the amplified product was determined. Homologous analysis of the ureA against that reported by Clayton CL and the GenBank and SwissProt databases were performed with the BLAST program at the Genome Net through the Internet. 0.8 kb PCR product was amplified from all H.pylori clinical isolators. The nucleotide sequence of the ureA was determined. The nucleotide sequence of the ureA began with ATG as the initiation codon and terminated in TAA as stop codon. The coding regions had a 44 % G C content. The DNA sequence was 98 % homologous to that reported by Clayton CL (688 out of 702 residues were identical). The derived amino acid sequences of the ureA were 99 % homologous to that reported by Clayton CL (232 out of 234 residues were identical). The nucleotide sequence and the predicted protein showed significant homology to ureA of H.pylori in the NCBI Entrez database. |
| Keywords: | Helicobacter pylori gene encoding urease subunit A clone PCR |
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