| HPLC法测定松花粉中山柰酚的含量 |
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| 引用本文： | 王正,顾蓉,朱彩芳,郦红岩.HPLC法测定松花粉中山柰酚的含量[J].齐鲁药事,2012,31(12):701-702. |
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| 作者姓名： | 王正 顾蓉 朱彩芳 郦红岩 |
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| 作者单位： | 江苏吉贝尔药业有限公司,江苏 镇江,212009 |
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| 摘 要： | 目的建立高效液相色谱法测定松花粉中山柰酚的含量。方法 Kromasil C18(4.6 mm×250 mm,5μm);流动相:甲醇-0.4%磷酸溶液(65∶35);检测波长:360 nm,柱温:30℃。结果山柰酚在0.124 4～1.989 6μg范围内呈良好的线性关系,平均加样回收率为99.6%,RSD为0.634%。结论该方法准确,重复性好,可用于该产品的质量控制。
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| 关 键 词： | 松花粉 山柰酚 含量测定 高效液相色谱法 |
Determination of kaempferol in Pine Pollen by HPLC |
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| WANG Zheng
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GU Rong
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ZHU Cai-fang
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LI Hong-yan.Determination of kaempferol in Pine Pollen by HPLC[J].qilu pharmaceutical affairs,2012,31(12):701-702. |
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| Authors: | WANG Zheng GU Rong ZHU Cai-fang LI Hong-yan |
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| Institution: | (Jiangsu Jibrier Pharmaceutical Co.,Ltd.,Zhenjiang 212009,China) |
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| Abstract: | Objective To establish an HPLC method for the determination of kaempferol in Pine Pollen.Methods The Kromasil C18 column(4.6 mm×250 mm,5 μm) was used and the mobile phase was composed of methanol-0.4%solution(65∶35).The detection wavelength was 360 nm and the column temperature was 30 ℃.Results The linear range was 0.124 4~1.989 6 μg,RSD=0.634% and the recovery of kaempferol was 99.6%.Conclusion This method was accurate and reproducible,and it can be used for the quality control of Pine Pollen. |
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| Keywords: | Pine Pollen Kaempferol Determination HPLC |
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