| PCR诊断犬感染细粒棘球绦虫方法检出日期的确定 |
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| 引用本文： | 张亚楼,马嵋,黄河,苗玉清,温浩.PCR诊断犬感染细粒棘球绦虫方法检出日期的确定[J].中国病原生物学杂志,2010(3). |
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| 作者姓名： | 张亚楼 马嵋 黄河 苗玉清 温浩 |
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| 作者单位： | 新疆医科大学一附院医学研究中心; |
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| 基金项目： | 国家高技术研究发展计划(863计划)项目(No.2007AA02Z411) |
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| 摘 要： | 目的确定犬细粒棘球绦虫感染PCR检测的窗口期。方法家犬8只,口服喂饲细粒棘球绦虫原头节,感染后40 d,收集犬粪,用PCR方法检测粪便中的目的DNA。结果6只犬获得细粒棘球绦虫重度感染,感染后21 dPCR检出目的DNA片段。结论犬重度感染细粒棘球绦虫后粪便PCR检测的窗口期为20 d。
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| 关 键 词： | 细粒棘球绦虫 粪源聚合式酶链反应 检测 窗口期 |
Determination of the date of PCR diagnosis of canine Echinococcus granulosus infection |
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| ZHANG Ya-lou,MA Mei,HUANG He,MIAO Yu-qing,WEN Hao.Determination of the date of PCR diagnosis of canine Echinococcus granulosus infection[J].Journal of Pathogen Biology,2010(3). |
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| Authors: | ZHANG Ya-lou MA Mei HUANG He MIAO Yu-qing WEN Hao |
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| Institution: | ZHANG Ya-lou,MA Mei,HUANG He,MIAO Yu-qing,WEN Hao(Medical Research Center,First Teaching Hospital of Xinjiang Medical University,Urumqi 830054,China) |
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| Abstract: | Objective To identify the date(window period) of diagnosis of dogs infected with Echinococcus granulosus by PCR detection.Methods Eight dogs were artificially infected with protoscoleces.Forty days later,their feces were collected and target DNA was detected by PCR.Results Only six dogs developed severe infections.Twenty-one days later,PCR detected target DNA fragments.Conclusion The date of PCR detection of a severe infection of E.granulosus was determined in dogs. |
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| Keywords: | Echinococcus granulosus copro-PCR detection window period |
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