| 口服基因疫苗预防柯萨奇B组病毒性心肌炎的实验研究 |
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| 引用本文： | 朱莉,刘晶星,陈洪,陈淑云,陆德源.口服基因疫苗预防柯萨奇B组病毒性心肌炎的实验研究[J].现代免疫学,2000,20(6):324-325. |
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| 作者姓名： | 朱莉 刘晶星 陈洪 陈淑云 陆德源 |
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| 作者单位： | 上海第二医科大学微生物学教研室,上海,200025 |
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| 基金项目： | 国家自然科学基金资助项目(39970691) |
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| 摘 要： | 为研制防治病毒性心肌炎的口服基因疫苗 ,本文以减毒鼠伤寒杆菌SL72 0 7作载体 ,将表达CVB3VP1基因的pcDNA3 VP1重组质粒转化入该细菌中 ,制备成口服基因疫苗。经口服免疫小鼠后 ,检测其免疫效果 ,并用CVB3攻击小鼠 ,观察其免疫保护作用 ,结果表明小鼠经 1次口服免疫后 ,血液中即产生了高滴度的抗体 ;病毒攻击后 ,免疫小鼠产生了明显的保护作用。
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| 关 键 词： | 柯萨奇B组病毒 VP1 口服基因疫苗 减毒鼠伤寒杆菌 |
| 文章编号： | 1001-2478(2000)06-0324-02 |
| 修稿时间： | 2000-03-28 |
A Preliminary Study on the Oral Transgene Vaccine of CVB3 Using Attenuated S.typhimurium |
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| Zhu Li,Liu Jingxing,Chen Hong,Chen Shuyun,Lu Deyuan.A Preliminary Study on the Oral Transgene Vaccine of CVB3 Using Attenuated S.typhimurium[J].Current Immunology,2000,20(6):324-325. |
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| Authors: | Zhu Li Liu Jingxing Chen Hong Chen Shuyun Lu Deyuan |
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| Institution: | Zhu Li
,Liu Jingxing
,Chen Hong
,Chen Shuyun
,Lu Deyuan
;(Department of Microbiology, Shanghai Second Medical University, Shanghai 200025) |
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| Abstract: | In order to study the oral gene vaccines against viral myocarditis,we used an attenuated strain of S typhimurium as a vehicle for oral genetic immunization Eukaryotic expression vectors containing CVB3 VP1 gene have been used to transform S typhimurium aroA,single oral immunization with such transformants induced excellent humoral responses.In addition,protective immunity was also induced with these transformants |
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| Keywords: | Coxsackievirus B VPl oral gene vaccines attenuated S Typhimurium |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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