硒酸精氨酸对D-gal衰老小鼠细胞免疫功能的影响

目的：探讨硒酸精氨酸对D-gal衰老小鼠免疫功能的影响。方法：将试验小鼠分为正常组、模型组、低剂量给硒组（L-SeArg）和高剂量给硒组（H-SeArg），通过后颈背部皮下注射D-gal建立亚急性衰老模型，以灌胃方式加入硒酸精氨酸，观察硒酸精氨酸对脾指数、胸腺指数的影响，MTT法检测脾细胞增殖能力和NK细胞的杀伤活性、免疫荧光法观察CD40、CD40L的阳性表达率。结果：硒酸精氨酸能明显提高小鼠胸腺指数和脾指数，促进淋巴细胞转化，提高NK细胞的杀伤活性，增加CIM0、CD40L的阳性表达率。2个剂量给硒组中，低剂量硒酸精氨酸的影响最大，与模型组相比有统计学意义（P〈0．01）。结论：D-gal能导致小鼠细胞免疫功能下降，硒酸精氨酸能活化免疫细胞，提高小鼠机体的免疫力，延缓衰老。

Effect of seleno-argnine on cellular immunological function in D-gal aging mice

AIM:To study the effect of seleno-argnine on cellular immunological function in D-gal aging mice.METHODS:The mice were divided into normal group,model group,low seleno-argnine group(L-SeArg) and high seleno-argnine group(H-SeArg).D-galactose induced aging model was set up by s.c.injection for 42 days,i.g.method was used to add Seleno-argnine.Spleen index(SI) and thymus index(TI) were measured.The ability of Spleen cell multiplication and NK cell killing activity were determined by MTT colorimetry.The changes of CD40 and CD40L expression were observed by immunofluorescent assay.RESULTS:Compared with the model mice,seleno-argnine increased spleen index and thynus index,enhanced the ability of spleen cell multiplication,enhanced the expression of CD40 and CD40L,and accelerated the NK cell killing activity.Between the two seleno-argnine groups,the effect of L-SeArg on cellular immunological function was more obvious in D-gal aging mice.The result were statistic ally significantly compared with the model mice(P<0.01).CONCLUSION:D-gal injection can decrease the cellular immunological function of mice.Seleno-argnine can activate immune cells,enhance the cellular immunological function of mice,reduce the toxic effect of D-gal and delay the aging process.