A rapid and highly predictive in vitro assay for non-steroidal anti-inflammatory agents |
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| Authors: | F Luzzani P Ventura G Zuccari G Clavenna |
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| Affiliation: | Analytical Chemistry Department, Piacenza, Italy. |
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| Abstract: | The inhibition of the production of malonyldialdehyde (MDA) in guinea-pig lung homogenates, incubated in the presence of 50 microM arachidonic acid and 1.4 mM adrenaline, has been exploited as a simple and reliable assay to test in vitro non-steroidal anti-inflammatory agents (NSAIA). The inhibitory potencies of a series of reference NSAIA, which correlated fairly well with in vivo anti-inflammatory activity as determined by carrageenin oedema, are herewith reported. The specificity of the assay was also evaluated by testing up to forty miscellaneous drugs: none of these significantly reduced the MDA production. |
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