HPLC法测定一点红中克氏千里光碱的含量 |
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引用本文: | 宁娱,祝晨蔯,莫洁丽,李泳雪,林朝展.HPLC法测定一点红中克氏千里光碱的含量[J].中国药品标准,2014,15(4):269-272. |
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作者姓名: | 宁娱 祝晨蔯 莫洁丽 李泳雪 林朝展 |
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作者单位: | [1]广州中医药大学临床药理研究所,广州510405; [2]广东省食品药品检验所,广州510180 |
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基金项目: | 国家药典委员会国家药品标准提高暨2015版药典一部“一点红质量标准研究”专题(任务书编号:Z-24) |
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摘 要: | 目的:建立高效液相色谱法测定一点红中克氏千里光碱含量的方法。方法:用Kromasil RP-C18色谱柱(4.6 mm ×250 mm,5μm),流动相为乙腈-水-磷酸-三乙胺(15∶85∶0.1∶0.4),检测波长为220 nm。结果:克氏千里光碱在10.04~200.80μg· mL-1范围内与峰面积呈良好线性关系(r=0.9998),平均加样回收率为96.34%,RSD为1.80%(n=6)。结论:此法快速、简便,专属性好,可用于一点红药材中克氏千里光碱的限量检查,为2015年版《中国药典》拟增订药材一点红的质量控制奠定基础。
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关 键 词: | 一点红 克氏千里光碱 含量测定 高效液相色谱法 |
收稿时间: | 2013/11/13 0:00:00 |
修稿时间: | 2014/6/19 0:00:00 |
Determination of Senkirkine in Herba Emiliae by HPLC |
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Authors: | Ning Yu Zhu Chenchen Mo Jieli Li Yongxue and Lin Chaozhan |
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Institution: | Ning Yu,Zhu Chenchen,Mo Jieli,Li Yongxue,Lin Chaozhan(1. Institute of Clinical Pharmacology, Cuangzhou University of Chinese Medicine, Guangzhou 510405 ; 2. Guangdong Institute for Food and Drug Control, Guangzhou 510180) |
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Abstract: | Objective:To establish an HPLC method for the determination of senkirkine in Herba Emiliae.Methods:The detection was performed on a Kromasil RP-C18 column(4.6 mm ×250 mm,5 μm) at UV wavelength of 220 nm.The mobile phase consisted of acetonitrile, water, phosphoric acid and triethylamine (15∶85∶0.1∶0.4 ).Results: The calibration curve was linear in the range of 10.04-200.80 μg· mL-1 of senkirkine ( r=0.9998 ).The average recovery was 96.34% with RSD of 1.80% ( n=6 ).Conclu-sion:The HPLC method is simple , rapid and accurate for determining senkirkine in Herba Emiliae which can be used for limit detec-tion of senkirkine in Herba Emiliae.The developed method was served for the quality evaluation of Herba Emiliae. |
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Keywords: | Herba Emiliae senkirkine determination HPLC |
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