铁缺乏对大鼠维生素A代谢的影响

目的研究铁缺乏对大鼠维生素A(VA)代谢的影响。方法44只雄性SD大鼠按体重随机分为4组,每组11只,正常对照组(Ⅰ组),Fe完全缺乏VA正常组(Ⅱ组),Fe轻度缺乏VA正常组(Ⅲ组),Fe和VA轻度缺乏组(IV组)。喂饲10周后处死,测定血清视黄醇、血清视黄醇结合蛋白(RBP)、血红蛋白(Hb)、血清铁、血清铁饱和度、肝脏VA含量,肝脏视黄基酯含量,并用逆转录-聚合酶链反应(RT-PCR)法检测各组大鼠肝脏RBP mRNA的表达。结果与对照组相比,铁缺乏可以使血清视黄醇、肝脏VA含量显著降低(P<0.05),使肝脏视黄基酯与肝脏VA的摩尔比值升高,使RBP含量有降低趋势,铁缺乏时肝脏RBP mRNA表达显著降低。结论铁缺乏可能通过影响维生素A吸收、储存、转运来影响体内维生素A的营养状况。

Effect of iron deficiency on vitamin A metabolism in rats

Objective To study the effect of iron deficiency on vitamin A (VA) metabolism in rats. Methods Forty-four male SD rats were divided randomly into four groups according to their body weights and every group included 11 rats: control group (Ⅰ group), entire iron deficient and normal VA group (Ⅱ group), slight iron deficient and normal VA group (Ⅲ group), slight iron and VA deficient(Ⅳ group). After being raised for 10 weeks, the rats were sacrificed and the serum VA, retinol-binding protein(RBP), hemoglobin, serum iron, serum iron saturation, VA in the liver, retinyl ester in the liver were determined. Results Iron deficiency decreased serum VA and VA in the liver significantly, and increased molar ratio of hepatic retinyl esters to retinol significantly. VA deficiency decreased the level of RBP mRNA expression in the liver significantly. Conclusion Iron deficiency could affect VA nutritional status by influencing absorption, storage and transportation of VA.