幽门螺杆菌诱导胃粘膜上皮细胞凋亡和增殖与p53基因表达

Apoptosis and proliferation induced by Helicobacter pylori and its assoc iation with p53 protein expression in gastric epithelial cells

AIM To study the relationship between gastric epithelial apoptosis and proliferation induced by Helicobacter pylori and p53 protein expression in H. pylori.related gastritis, analyze the mechanisms of H. pylori infection induced gastric epithelial cell apoptosis as well as proliferation, and explore the role of H. pylori in the development of gastric diseases. METHODS Endoscopic mucosal biopsies were taken in 72 cases of chronic gastritis. Using terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) technique and immunohistochemical staining, we observed the apoptotic cells and proliferative cells in situ in H. pylori negative gastric mucosa, H. pylori positive gastric mucosa before and after H. pylori eradication, and compared the number of apoptotic cells and proliferative cells with the p53 gene expression in each case. RESULTS The apoptotic index and PCNA index of gastric epithelial in the patients infected with H. pylori (12.7% and 14.1%) were significantly higher than those of H. pylori negative gastric epithelial (3.0% and 8.3%, t=7. 241,6.368, P