| 用MCF-7细胞检测多氯联苯拟雌激素样活性 |
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| 引用本文： | 董力,张明.用MCF-7细胞检测多氯联苯拟雌激素样活性[J].职业与健康,2008,24(10):923-924. |
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| 作者姓名： | 董力 张明 |
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| 作者单位： | 中国疾病预防控制中心环境与健康相关产品安全所,北京市,100050 |
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| 摘 要： | 目的观察多氯联苯（PCB）对MCF-7细胞增殖及生长曲线的影响。方法选用多氯联苯（1254）进行MCF-7人乳腺癌细胞增殖实验。结果PCB组细胞增殖指数均明显高于溶剂对照组，10ng／LPCB可诱导MCF-7细胞最大增殖（P〈Q01）。生长曲线分析也显示，PCB、E2生长曲线明显上移，有明显刺激MCF-7细胞增殖的类雌激素活性。结论提示PCB具有拟雌激素样活性。
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| 关 键 词： | 雌激素样活性 人乳腺癌细胞增殖实验 多氯联苯 |
| 文章编号： | 1004-1257(2006)10-0923-02 |
| 修稿时间： | 2007年11月13 |
Application of MCF-7 Cells in the Detection of Polychlorobiphenyl Imitative Estrogenic Activity |
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| DONG Li,ZHANG Ming.Application of MCF-7 Cells in the Detection of Polychlorobiphenyl Imitative Estrogenic Activity[J].Occupation and Health,2008,24(10):923-924. |
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| Authors: | DONG Li ZHANG Ming |
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| Institution: | (Institute of Environmental Health and Related Products Safety, China Center for Disease Control and Prevention, Beijing, 100050, China ) |
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| Abstract: | Objective] To observe the effect of polychlorobiphenyl on the multiplication and growth curve of MCF 7 cells. Methods] Polychlorobiphenyl was used to conduct multiplication experiment on MCF 7 mammary carcinoma cells. Results] The cells multiplication index of the polychlorobiphenyl group was significantly higher than that of the control; 10 ng/L polychlorobiphenyl could induce the maximum multiplication of MCF 7 cells （P〈0. 01）; the growth curve of polychlorobiphenyl and E2 increased significantly, which obviously stimulated imitative estrogen activity of the cell proliferation. Conclusion] It is indicated that polychlorobiphenyl has imitative estrogen activity. |
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| Keywords: | Estrogenic activity Multiplication experiment of mammary carcinoma cells Polychlorobiphenyl |
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