| 抑郁模型大鼠杏仁核神经元细胞色素C和Bax的表达 |
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| 引用本文： | 田艳霞,王海涛,李冉,阚泉,高俊玲.抑郁模型大鼠杏仁核神经元细胞色素C和Bax的表达[J].中国煤炭工业医学杂志,2010,13(8):1202-1203. |
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| 作者姓名： | 田艳霞 王海涛 李冉 阚泉 高俊玲 |
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| 作者单位： | 华北煤炭医学院组织学和胚胎学教研室,河北省唐山市063000 |
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| 摘 要： | 目的观察细胞色素C（CytC）和凋亡相关蛋白Bax在抑郁模型大鼠杏仁核神经元的表达。方法将成年健康雄性Wistar大鼠随机分为对照组和模型组,每组15只。采用慢性强迫游泳（4周）制备慢性强迫游泳应激抑郁模型。采用TUNEL和流式细胞术检测杏仁核神经元凋亡和凋亡率,Western Blot检测CytC和Bax蛋白的表达。结果模型组和对照组凋亡细胞阳性率分别为（24.08±4.30）%和（3.08±0.91）%,凋亡率分别为（17.14±2.71）%和（3.34±0.80）%,凋亡相关蛋白Bax以及CytC蛋白明显高于对照组。结论抑郁模型大鼠杏仁核存在明显的神经元凋亡,Bax上调和CytC释放可能是抑郁患者杏仁核神经元凋亡的机制之一。
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| 关 键 词： | 慢性强迫游泳应激 杏仁核 凋亡 细胞色素C Bax 大鼠 |
EXPRESSION OF CYTOCHROME C AND BAX IN AMYGDALA NEUROS OF DEPRESSION MODEL RATS |
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| Institution: | Tian Yanxia,Wang Haitao,Li Ran,et al.Department of Histology and Embryology,North China Coal Medical college,Tangshan 063000,China |
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| Abstract: | Objective To observe the expression of Cytochrome C and Bax in amygdala of depression model rats.Methods Male Wistar rats were randomly divided into control group and model group. Chronic forced swimming stress was carried out to set up the rat depression model equally. TUNEL-staining and double-labeled flow cytometry （FCM） were employed for the detection and quantification of the apoptotic cells in the amygdala. The expression of Cytochrome C and Bax was detected using Western blotting. Results The percentage of TUNEL-positive cell,the apoptotic cell rate and the expression of Cytochrome C and Bax of model group were higher than those of control group respectively （P0.01）. Conclusion Apoptosis is increased in amygdala neurons of depression model rats,and expression bax protein is up-regulated and promotes the releasing of Cytochrome C. |
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| Keywords: | chronic forced swimming stress amygdala apoptosis Cytochrome C bax rat |
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