| 免疫介导再生障碍性贫血小鼠IL-3和IL-3受体变化的研究 |
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| 引用本文： | 黄韬,黄振翘,周永明,薛志忠,甘人宝. 免疫介导再生障碍性贫血小鼠IL-3和IL-3受体变化的研究[J]. 中国实验血液学杂志, 2004, 12(5): 684-686 |
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| 作者姓名： | 黄韬 黄振翘 周永明 薛志忠 甘人宝 |
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| 作者单位： | 1. 上海中医药大学附属岳阳中西医结合医院血液科,上海,200437
2. 中国科学院上海生物化学研究所,上海,200031 |
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| 摘 要： | 本研究目的是为再生障碍性的贫血的临床治疗提供新的思路。建立了免疫介导再生障碍性贫血小鼠模型 ,用酶联免疫吸附法 (ELISA)测定植物血凝素 (PHA)刺激的再生障碍性贫血小鼠脾细胞培养上清液中IL 3,以点杂交法对其靶细胞受体进行半定量分析。结果表明 ,用植物血凝素 (PHA)刺激的再生障碍性小鼠脾细胞培养上清液中IL 3含量高于正常对照 ,二者之间有明显差异 (P <0 .0 0 1) ;其靶细胞IL 3受体的表达量明显低于正常对照。结论 :IL 3靶细胞的IL 3受体水平在再生障碍性贫血的治疗中起着重要的作用。
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| 关 键 词： | 免疫 再生障碍性贫血 动物模型 IL-3 IL-3R |
| 文章编号： | 1009-2137(2004)05-0684-03 |
| 修稿时间： | 2003-10-20 |
Study on the Changes of IL-3 and Its Receptor in Mice with Immune-mediated Aplastic Anemia |
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| HUANG Tao,HUANG Zhen-Qiao,ZHOU Yong-Ming,XUE Zhi-Zhong,GAN Ren-B ao. Study on the Changes of IL-3 and Its Receptor in Mice with Immune-mediated Aplastic Anemia[J]. Journal of experimental hematology, 2004, 12(5): 684-686 |
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| Authors: | HUANG Tao HUANG Zhen-Qiao ZHOU Yong-Ming XUE Zhi-Zhong GAN Ren-B ao |
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| Affiliation: | Department of Hematology, The Yue Yang Iraditional Chinese and Weseten Medicine Integrated Hospital Affiliated to Shanghai Traditional Chinese University, Shanghai 200437, China. 13331993136@133sh.com |
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| Abstract: | The aim of this study was to find new idea for cl inical treatment of aplastic anemia. Immune-mediated aplastic anemia mice were developed, IL-3 in the supernatant with PHA stimulating splenic cells was det ected by ELISA, semi-quantiting analysis of IL-3R was performed by point hybri dization.The results showed that the IL-3 level in the supernatant with PHA stimulating splenic cells of immune-mediated aplastic anemia mice was high er than controls, difference between them was significant (P<0.001), while a mount of IL-3 receptor by semi-quantiting analysis was lower than control sig nificantly. In conclusion, the IL-3 receptor expression level is important for pathogenesis and treatment strategy of aplastic anemia. |
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| Keywords: | immune aplastic anemia animal model IL-3 IL -3R |
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