应用FISH技术检测肺腺癌,鳞癌细胞部分染色体数量的改变

目的研究肺癌细胞７号，１７号及Ｙ染色体的数目改变及其意义。方法应用荧光原位杂交技术（ＦＩＳＨ）技术，对１７例腺癌和鳞癌的细胞印片进行检测。结果７号染色体：三体细胞占细胞总数的３１．７３％。１７号染色体：二体细胞占细胞总数的４３％。Ｙ染色体：单体细胞占细胞总数的５８．０４％。结论在肺腺癌和肺鳞癌中，７号染色体数目均有明显增加，并以三体所占比例最高。肺腺癌与肺鳞癌７号染色体数目分布差异明显。

Numerical change of 7,17 and Y chromosomes in human lung carcinoma detected by fluorescence in situ hybridization

Ojbective To study the copy number of chromosomes7,17 and Y in lung carcinomas. Methods Fluorescence in situ hybridization (FISH) was used to detect the number of above mentioned chromosomes on the touch smears of 17 cases of lung carcinoma (adenocarcinoma and squamous cell carcinoma). Results Triploid chromosome 7 FISH signals were found in 31.73% of the tumor nuclei,dipoid chromosome 17 FISH signals in 43.00% and monoploid chromosome Y FISH signals in 58.04% of these nuclei. Conclusions The number of chromosome 7 was obviously increased in both lung adenocarcinoma and squamous cell carcinoma, and the rate of trisomy 7 was the highest. In addition,there is a significant difference in the distribution of chromosome 7 ploidy between lung adenocarcinoma and squamous cell carcinoma.