CD4^＋CD25^＋CD127low调节性T细胞及相关细胞因子在儿童再障发病中的作用

目的探讨CD4^＋CD25^＋CD127low调节性T细胞及转化生长因子-β1（TGF-β1）在再障患儿外周血中的表达及临床意义。方法采用流式细胞术分别检测21例慢性再障（CAA）、9例急性再障（SAA）和15例正常健康儿童外周血自细胞，并用酶联免疫吸附法检测其外周血TGF-β1表达水平。结果外周血中CD；T细胞百分比、CD4^＋CD25^＋,CD4^＋CD25^＋CD127low调节性T细胞占CD4^＋T细胞百分比及血清TGF-β1表达水平再障患儿均低于正常健康儿童（P均〈0．05），S从低于CAA（P均〈0．05）；再障患儿CD4^＋CD25^＋CD127low调节性T细胞占CD4^＋T细胞百分比与TGF-β1的表达水平无明显相关性。结论再障患儿周血调节性T细胞比例减少、血清TGF-β1表达水平减低可能参与了再障的发病。

Roles of CD_4~+CD_(25)~+CD_(low)~(127) regulatory T cells and related cytokine in children with aplastic anemia

Objective To explore the levels of CD4^＋CD25^＋CD127low regulatory T cells （Tregs） and TGF-β1 in peripheral blood of aplastic anemia （AA） in children, and to investigate the role of Tregs in the pathogenesis of aplastic anemia. Methods A total of 21 patients with chronic aplastic anemia （CAA）, 9 patients with acute aplastic anemia （SAA） and CD4^＋CD25^＋CD127low T ceils in CD4＋ T cells was evalu-15 normal healthy children were included in this study. The proportion of CD4＋CD25^＋CD127^low ated by flow cytometric analysis. TGF-β1 was measured by ELISA. Results The percentage of CD4＋ T cells, CD4＋CD25^＋ T cells. Tregs in peripheral blood and serum TGF-β1 expression in patients with AA were lower than those in the normal control （all P 〈 0.05）. The percentage mentioned above in SAA was lower than that in CAA （all P 〈 0.05 ）. Tregs percentage and the level of TGF-β1 in children with AA were no significant correlation. Conclusions The low percentage of regulatory T cells and the low level of TGF-β1 in children may be involved in pathophysiology of AA.