Diversity of intrathecal antibody synthesis against HTLV-I and its relation to HTLV-I associated myelopathy |
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Authors: | Bernd Kitze Koichiro Usuku Shuji Izumo Minoru Nakamura Hiroshi Shiraki Shinji Ijichi Shinji Yashiki Toshinobu Fujiyoshi Shunro Sonoda Mitsuhiro Osame |
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Institution: | (1) Department of Neurology, Göttingen University, Robert-Koch-Strasse 40, D-37075 Göttingen, Germany;(2) Center for Chronic Viral Diseases, Kagoshima University, Kagoshima, Japan;(3) Third Department of Internal Medicine, Kagoshima University, Kagoshima, Japan;(4) Department of Virology, Kagoshima University, Kagoshima, Japan;(5) First Department of Internal Medicine, Kyushu University, Fukuoka, Japan;(6) Fukuoka Red Cross Blood Center, Fukuoka, Japan |
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Abstract: | The humoral immune response against human T-cell lymphotropic virus type I (HTLV-I) in the central nervous system (CNS) compartment and in the blood was investigated by enzyme immunoassay using 16 synthetic peptides corresponding to HTLV-I core and envelope sequences. We evaluated paired samples of cerebrospinal fluid and serum from HTLV-I seropositive Japanese patients, classified as follows: HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP;n = 39), patients with spinal cord disease ascribed to either HAM/TSP or to some concomitant, HTLV-I-unrelated disease (possible HAM/TSP;n = 6) or carriers without any clinical signs of HAM/TSP (n = 15). HTLV-I-peptide-specific intrathecal antibody synthesis was found in 79% of HAM/TSP patients, but only in 20% of carriers without HAM/TSP. The group of carriers without HAM/TSP showed local synthesis for some peptides (on average 0.3 peptides per patient). In most HAM/TSP patients, however, there was a diverse intrathecal immune response to several HTLV-I synthetic peptides (on average against 3.6 peptides per HAM/TSP patient), most frequently againstgag p19 100–130,env gp21 458–488, andenv gp46 175–199 and 288–317. The intrathecal antibody synthesis against several HTLV-I determinants may represent a pathogenic immune response in HAM/TSP and is possibly related to the infiltration of virus-infected T-cells in the spinal cord. |
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Keywords: | Human T-cell lymphotropic virus type I associated myelopathy/tropical spastic paraparesis Virus-specific antibodies Immunoglobulin G Intrathecal antibody formation |
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