| 白术组培快繁技术 |
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| 引用本文： | 朱玉球,夏国华,方慧刚,付顺华,何福基. 白术组培快繁技术[J]. 中药材, 2006, 29(3): 212-213 |
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| 作者姓名： | 朱玉球 夏国华 方慧刚 付顺华 何福基 |
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| 作者单位： | 浙江林学院生命科学学院,浙江,临安,311300 |
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| 摘 要： | 白术外植体诱导及植株再生试验结果表明:NAA为白术叶片、叶柄愈伤组织诱导和芽分化的主导因子;MS BA 1.0 mg/L NAA 0.3 mg/L GA30.2 mg/L为白术叶片、叶柄愈伤组织诱导、芽分化的最佳培养基;腋芽增殖的培养基以MS BA 0.3 mg/L IBA 0.5 mg/L较为理想,诱导率达95%;1/2MS IBA 0.1~0.5 mg/L为白术试管苗生根的最佳培养基,生根率在90%以上;试管苗移栽成活率达90%以上。
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| 关 键 词： | 白术 组织培养 快速繁殖 |
| 文章编号： | 1001-4454(2006)03-0212-02 |
| 收稿时间： | 2005-07-15 |
| 修稿时间： | 2005-12-08 |
Study on Tissue Culture and Rapid Propagation of Atractylodes macrocephala |
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| ZHU Yu-qiu,XIA Guo-hua,FANG Hui-gang,FU Shun-hua,HE Fu-ji. Study on Tissue Culture and Rapid Propagation of Atractylodes macrocephala[J]. Journal of Chinese medicinal materials, 2006, 29(3): 212-213 |
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| Authors: | ZHU Yu-qiu XIA Guo-hua FANG Hui-gang FU Shun-hua HE Fu-ji |
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| Affiliation: | Faculty of Forestry and Biotechnology, Zhejiang Forestry University, Lin'an, Zhejiang 311300, China. |
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| Abstract: | A study was conducted by tissue culture on the explant of Atractylodes macrocephala Koidz..The result showed that NAA was a leading factor for induction of callus from leaf blades and the petiole as well as bud differentiation.MS BA 1.0 mg/L NAA 0.3 mg/L GA_3 0.2 mg/L was an optimal medium for induction of callus from leaf blades and the petiole and for the bud differentation also.MS BA 3.0 mg/L IBA 0.5 mg/L was suitable for the proliferation of axillary buds,with an induction rate of 95%.1/2MS IBA 0.1~0.5 mg/L was optimum for rooting,with a rooting percentage more than 90%.The survival rate of transplanted plantlets was more than 90%. |
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| Keywords: | Atractylodes macrocephala Koidz. Tissue culture Rapid propagation |
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