| C57BL／6-gfp小鼠嗅球神经干细胞的培养鉴定及耳蜗核移植 |
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| 引用本文： | 陈阳,王枫,陈俊,高雪,米文娟,邱建华. C57BL／6-gfp小鼠嗅球神经干细胞的培养鉴定及耳蜗核移植[J]. 中国耳鼻咽喉头颈外科, 2009, 0(1): 7-9 |
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| 作者姓名： | 陈阳 王枫 陈俊 高雪 米文娟 邱建华 |
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| 作者单位： | 第四军医大学西京医院全军耳鼻咽喉头颈外科中心,陕西西安710032 |
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| 基金项目： | 基金项目：国家自然科学基金面上项目（30572023,30600703） |
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| 摘 要： | 目的建立C57BL／6－gfp小鼠嗅球神经干细胞体外培养的方法，并初步应用于大鼠耳蜗核定位移植。方法培养C57BL／6－gfp小鼠胚胎嗅球神经千细胞，传代并进行分化实验及鉴定后将其立体定位注射于大鼠耳蜗核。结果所培养的神经干细胞生长良好，可稳定传代，能够分化为3种神经细胞。定位注射后可在局部见到绿色荧光阳性的细胞团。结论该方法培养的C57BL／6-gfp小鼠胚胎嗅球神经干细胞可稳定传代并可以作为荧光标记细胞进行移植实验。
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| 关 键 词： | 荧光抗体技术 小鼠 转基因 干细胞 细胞移植 耳蜗 |
Culturing C57BL/6-gfp mouse olfactory bulb neural stem cells and transplantation in cochlear nucleus |
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| CHEN Yang,WANG Feng,CHEN Jun,GAO Xue,MI Wenjuan,QIU Jianhua. Culturing C57BL/6-gfp mouse olfactory bulb neural stem cells and transplantation in cochlear nucleus[J]. Chinese Archives of Otolaryngology-Head and Neck Surgery, 2009, 0(1): 7-9 |
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| Authors: | CHEN Yang WANG Feng CHEN Jun GAO Xue MI Wenjuan QIU Jianhua |
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| Affiliation: | ( Department of Otolaryngology Head and Neck Surgery, Xijing Hospital, the Fourth Military Medical University, Xi＇an, Shanxi, 710032, China) |
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| Abstract: | OBJECTIVE To establish a stable method of culturing olfactory bulb neural stem cells from C57BL/6-gfp mouse, and to explore its application in the stereotactic injection in cochlear nucleus of rats. METHODS Olfactory bulb stem cells from C57BL/6-gfp mouse were cultured and passed down in vitro. The cells specificity were identified by differentiation and specific marker via immunohistochemical staining. Stereotactic injecting of cultured cells into the rat cochlear nucleus was performed. RESULTS Neurosphere-forming cells were isolated and collected with diluted purification method from C57BL/6-gfp mouse olfactory epithelium. These neurosphere-forming cells were nestin immuno- positive and differentiated into neurons, astrocytes and oligodendrocytes. CONCLUSION NSCs can be isolated and cultured in vitro from C57BL/6-gfp mouse olfactory bulb and be applied into stereotactic injection. |
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| Keywords: | Fluorescent Antibody Technique Mice, Transgenic Stem Cells Cell Transplantation Cochlea |
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