基质金属蛋白酶-9在乳腺癌组织中的表达及其临床意义

目的：研究乳腺癌组织中基质金属蛋白酶-9（MMP-9）的表达情况及其与乳腺癌转移和预后的相关性,寻找能够有效判断乳腺癌转移潜能的分子生物学指标.方法：应用组织芯片技术和免疫组化方法（PV-9000法）检测106例乳腺浸润性导管癌石蜡包埋组织中MMP-9的表达情况,分析MMP-9的表达与淋巴结转移、肿块直径、病理分级、临床分期、患者年龄及雌激素受体（ER）、孕激素受体（PR）、人表皮生长因子受体-2（HER-2）表达的关系,并分析MMP-9不同表达强度患者的生存率.结果：（1）106例乳腺癌组织中MMP-9表达阳性率为81.13%,随肿块直径、病理分级、临床分期的增加呈明显升高趋势,差异有统计学意义（P＜0.05）,腋淋巴结无转移者MMP-9阳性表达率（（71.4%）明显低于腋淋巴结有转移者（87.5%）（P＜0.05）;（2）乳腺癌组织MMP-9表达与ER、PR、Her-2的表达之间不存在关联关系（P＞0.05）.（3） MMP-9表达越强,患者术后生存率越低（P＜0.05）.结论：MMP-9的表达在乳腺癌淋巴结转移中起重要作用,MMP-9可作为乳腺癌预后不良的独立参数.

Expression of matrix metalloproteinase-9 (MMP-9) of breast carcinoma and its clinical significance by tissue chip

Objective: To investigate the expressions of matrix metalloproteinase 9 protein (MMP-9) in breast carcinoma cells and analyze their correlations, trying to find the molecular biological markers for evaluating the metastatic potentiality of the breast carcinoma. Methods: The paraffin-embedded specimens from 106 breast invasive ductal carcinoma patients being made to tissue chips were stained for detection of expressions of MMP-9 with the immunohistochemical method, studying correlations of expressions of MMP-9 in the breast carcinoma with lymph node metastasis, tumor sizes, pathological grading, et al. Result: (1) The positive expression rate of MMP-9 in 106 breast invasive ductal carcinoma tissue was 81.13%. The expressions of MMP-9 were correlated positively with tumor sizes, pathological grading, clinical staging (P<0.05, r=0.274, 0.327, 0.260). The expressions of MMP-9 in the group without lymph node metastasis (71.4%) were lower than the expressions of MMP-9 in the group with lymph node metastasis (87.5%),the two groups had significant difference (P<0.05). The expressions of MMP-9 were no correlations with the expressions of ER, PR, HER-2 (P>0.05). (3) The higher the positive expressions of MMP-9 were, the patient survival rates were lower (P<0.05). Conclusion: The expression of MMP-9 plays an important role in the metastasis of lymph node in breast carcinoma. MMP-9 can be an independent prognostic indicator to breast carcinoma.