Macrophage activating activity of pyrrole alkaloids from Morus alba fruits |
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Authors: | Seon Beom Kim Bo Yoon Chang Yang Hee Jo Sang Hoon Lee Sang-Bae Han Bang Yeon Hwang Sung Yeon Kim Mi Kyeong Lee |
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Affiliation: | 1. College of Pharmacy, Chungbuk National University, Cheongju 361-763, Republic of Korea;2. Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Chonbuk 570-749, Republic of Korea |
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Abstract: | Ethnopharmacological relevanceThe fruits of Morus alba have been traditionally used as a tonic to enhance immune responses.Materials and methodsThe macrophage activating constituents of Morus alba fruits were purified using various column chromatography techniques. The structures of isolated compounds were determined on the basis of spectroscopic data interpretation such as 1D and 2D NMR analysis. The macrophage activating activities of isolated compounds were evaluated by measuring the production of nitric oxide, TNF-α and IL-12 in RAW 264.7 cells. The phagocytic activity was also evaluated.ResultsFive pyrrole alkaloids, 5-(hydroxymethyl)-1H-pyrrole-2-carboxaldehyde (1), 2-formyl-1H-pyrrole-1-butanoic acid (2), 2-formyl-5-(hydroxymethyl)-1H-pyrrole-1-butanoic acid (3), 2-formyl-5-(methoxymethyl)-1H-pyrrole-1-butanoic acid (4) and Morrole A (5) were isolated from the fruits of Morus alba. Morrole A (5) is first reported in nature and other pyrrole alkaloids (1–4) are first reported from Morus species. Among the isolated compounds, compounds 3 and 4 significantly activated macrophage activity by the enhancement of nitric oxide, TNF-α and IL-12 production, and the stimulation of phagocytic activity in RAW 264.7 cells.ConclusionPyrrole alkaloids, including a new compound, were isolated from Morus alba fruits. These compounds activated macrophage activity in RAW 264.7 cells. |
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Keywords: | Morus alba Moraceae Pyrrole alkaloid Morrole A Macrophage activation |
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