Mechanism of protective effects of Danshen against iron overload-induced injury in mice |
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Authors: | Yonggang Gao Na Wang Ying Zhang Zhihong Ma Peng Guan Juanjuan Ma Yuanyuan Zhang Xuejing Zhang Jiangyan Wang Jianping Zhang Li Chu |
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Institution: | 1. Department of Pharmacology, School of Basic Medicine, Hebei Medical University, 326, Xinshi South Road, Shijiazhuang, 050091 Hebei, China;2. The Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, Hebei Normal University, Shijiazhuang, 050016 Hebei, China;3. Department of Pathology, School of Basic Medicine, Hebei Medical University, 326, Xinshi South Road, Shijiazhuang, 050091 Hebei, China;4. Department of Biochemistry, Bethune Military Medical College, 450, Zhongshan Western Road, Shijiazhuang, 050081 Hebei, China |
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Abstract: | Ethnopharmacological relevanceDanshen (Salvia miltiorrhiza) has been widely prescribed in traditional folk medicine for treatment of hepatic and cardiovascular diseases in China and other Asian countries for several hundred years.Materials and methodsSixty male mice were randomly divided into five groups: control, iron overload, low-dose Danshen (L-Danshen, 3 g/kg/day), high-dose Danshen (H-Danshen, 6 g/kg/day) and deferoxamine (DFO) groups (n=12 per group). Iron dextran was injected intraperitoneally (i.p.) at 50 mg/kg body weight/day to establish the iron overload model. While control mice received saline, mice of the treated groups simultaneously received (i.p.) injections of L-Danshen, H-Danshen or DFO daily for 2 weeks. At the end of the experiment, changes in alanine aminotransferase (ALT) and aspartate aminotransferase (AST), glutathione peroxidase (GSH-Px), superoxide desmutase (SOD) and malondialdehyde (MDA) were measured, and histological changes were observed by Prussian blue or hematoxylin and eosin staining of the liver. Apoptosis was detected by terminal-deoxynucleotidyl transferase mediated nick end labeling.ResultsTreatment of iron overloaded mice with either low or high doses of Danshen not only significantly attenuated the hepatic dysfunction (ALT/AST levels), decreased the content of MDA and increased the activities of GSH-Px and SOD, it also suppressed apoptosis in hepatocytes. Histopathological examination showed that treatment with Danshen reduced iron deposition and ameliorated pathological changes in the liver of iron overloaded mice.ConclusionsDanshen demonstrated significant protective effects in the liver of iron overloaded mice, which were at least partly due to the decrease of iron deposition and inhibition of lipid peroxidation and hepatocyte apoptosis. |
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Keywords: | ALT alanine aminotransferase AST aspartate aminotransferase BSA bovine serum albumin BW body weight CCBs calcium channel blockers DFO deferoxamine GSH-Px glutathione peroxidase H-Danshen high-dose Danshen H& E hematoxylin and eosin i p intraperitoneally KM mice Kunming mice L-Danshen low-dose Danshen MDA malondialdehyde SOD superoxide desmutase TUNEL TdT-mediated dUDP nick end labeling |
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