Preparation and drug release mechanism of CTS-TAX-NP-MSCs drug delivery system |
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Authors: | Tian Dai Enyun Yang Yongjun Sun Linan Zhang Li Zhang Ning Shen Shuo Li Lei Liu Yinghua Xie Shaomei Wu Zibin Gao |
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Affiliation: | 1. Department of Pharmacy, Hebei University of Science and Technology, Yuhua East Road 70, Shijiazhuang 050018, PR China;2. Department of Pharmaceutics, New Drug Research and Development Center, North China Pharmaceutical Group Corporation, Heping East Road 388, Shijiazhuang 050015, PR China;3. State Key Laboratory Breeding Base—Hebei Province Key Laboratory of Molecular Chemistry for Drug, PR China |
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Abstract: | Targeting delivery of anticancer agents is a promising field in anticancer therapy. Inherent tumor-tropic and migratory properties of mesenchymal stem cells (MSCs) make them potential vehicles for targeting drug delivery systems for tumors. Although, MSCs have been successfully studied and discussed as a vehicle for cancer gene therapy, they have not yet been studied adequately as a potential vehicle for traditional chemical anticancer drugs. In this study, we have engineered MSCs as a potential targeting delivery vehicle for paclitaxel (TAX)-loaded nanoparticles (NPs). The size, surface charge, starving time of MSCs, incubating time and concentration of NPs could influence the efficiency of NPs uptake. In vitro release of TAX from CTS (chitosan)-TAX-NP-MSCs and the expression of P-glycoprotein demonstrated that release of TAX from MSCs might involve both passive diffusion and active transport. In vitro migration assays indicated that MSCs at passage number 3 have the highest migrating ability. Although, the migration ability of CTS-TAX-NP-MSCs could be inhibited by uptake of CTS-TAX-NPs, this ability could recover 6 days after the internalization. |
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Keywords: | Mesenchymal stem cells Vehicle Drug delivery Cancer Nanoparticle |
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