皮肤鳞状细胞癌Fas和FADD表达的差异性分析

目的：观察死亡受体（Fas）、Fas相关死亡结构域（Fas-associ ated death domain,FADD）在皮肤鳞状细胞癌（SCC）组织中的表达情况,分析其表达差异及意义。方法：应用免疫组织化学SABC法对27例SCC组织的石蜡切片及10例正常皮肤组织的切片分别进行Fas、FADD染色,在光学显微镜下观察并计数其阳性细胞,根据阳性细胞平均表达积分来判定。结果采用t检验和等级相关分析。结果：①Fas、FADD在10例正常皮肤组织中表达均为阳性,平均表达积分3.10±0.87、3.20±0.63;②Fas在27例SCC组织中的平均表达积分2.19±1.14,低于正常对照组,差异有统计学意义（P〈0.05）;FADD在27例SCC组织中的平均表达积分2.15±1.40,低于正常对照组,差异有统计学意义（P〈0.05）;③在27例SCC组织中,Fas、FADD蛋白表达程度与病理分级呈正相关,有统计学意义（P〈0.05）。结论：①Fas和FADD共同的低表达,可能参与了皮肤SCC的发生发展;②Fas、FADD蛋白的异常表达程度与皮肤SCC的恶性程度有关,同时检测两个蛋白的表达情况,有助于判断皮肤SCC的病理分级,有可能作为反映鳞状细胞癌预后的指标。

Difference analysis on expression of Fas and FADD in tissues of Squamous cell carcinoma

Objective To observe the expressions of Fas and FADD in Squamous cell carcinoma（SCC）,analyze difference and significance of expressions.Methods Fas and FADD were assayed by immunohistochemistry techniques（SABC） in specimens from 27 SCC and 10 normal skin,observed and accounted positive cells by optics microscope.According to the integrate of positive cells,the results were analyzed by T test and correlation analyse.Results ①Fas、FADD were both positive expression in normal skin tissue.②The expressions of Fas in SCC were lower than in normal skin tissue,which showed difference（P0.05）.The expressions of FADD in SCC were lower than in normal skin tissue,which showed difference（P0.05）;③The integrate of positive cells of Fas、FADD was positive correlation with tumor vicious degree of SCC,which showed difference（P0.05）.Conclusions The aberrant expression of Fas、FADD proteins played an important role in the pathogenesis and development of SCC.The higher the rates of these protein aberrant expressions were,the more malignancy SCC was,which was helpful for the pathological diagnosis of SCC,and Fas、FADD might be the index of refecting prognostic SCC.