| Bcl-2核酶对SMMC7721细胞的促凋亡机制 |
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| 引用本文： | 张传山,王文亮,彭玮丹. Bcl-2核酶对SMMC7721细胞的促凋亡机制[J]. 世界华人消化杂志, 2000, 8(4): 417-419 |
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| 作者姓名： | 张传山 王文亮 彭玮丹 |
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| 作者单位： | 710033,陕西省西安市,中国人民解放军第四军医大学病理学教研室. |
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| 摘 要： | 目的 观察Bel-2核酶对SMMC-7721细胞的作用，探讨bcl-2抑制细胞凋亡的机制。方法 经脂质体介导的方法将PMTr-neo(正向Bcl-2核酶真核表达载体)导入SMMC 7721细胞中．细胞克隆转移扩大培养后，采用TUNEL,TRAP结合ELISA流式细胞仪，免疫组化技术检测SMMC 7721／PMTr-neo细胞增殖及细胞凋亡。结果 较对照组SMMC 7721／PMTr-neo细胞bcl-2表达水平显著下降，伴有显著细胞凋亡现象及端粒酶活性下降，结论 Bcl-2核酶可促进SMMC 7721细胞发生凋亡，并降低细胞端粒酶活性，为反义技术在肝癌治疗中应用提供理论依据。
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| 关 键 词： | 肝肿瘤；bcl-2；核酶；细胞凋亡 |
| 修稿时间： | 1999-12-22 |
Promotion of apoptosis of SMMC7721 cells by Bcl-2 ribozyme |
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| Chuen Shan Zheng,Wen Liang WangWei Dan PengPeiZhen HuYu Bo Chai Fu Cheng Ma. Promotion of apoptosis of SMMC7721 cells by Bcl-2 ribozyme[J]. World Chinese Journal of Digestology, 2000, 8(4): 417-419 |
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| Authors: | Chuen Shan Zheng Wen Liang WangWei Dan PengPeiZhen HuYu Bo Chai Fu Cheng Ma |
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| Affiliation: | Chuen Shan Zheng,Wen Liang Wang;(Department of Pathology,University,Xian 710033,Shaanxi Province,China);Wei Dan Peng;(Department of Biochemistry,Fourth Military Medical University,Xian 710033,Shaanxi Province,China);PeiZhen Hu;(Department of Pathology,University,Xian 710033,Shaanxi Province,China);Yu Bo Chai;(Department of Biochemistry,Fourth Military Medical University,Xian 710033,Shaanxi Province,China); Fu Cheng Ma;(Department of Pathology,University,Xian 710033,Shaanxi Province,China) |
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| Abstract: | AIM To observe the effect of Bcl-2 ribozyme on SMMC 7721 cells and to investigate bcl-2 inhibition of cellular apoptosis.METHODS PMTr-neo (sense Bcl-2 ribozyme eucaryotic expression vector) was introduced into SMMC7221 cells by using lipofectin mediation; TUNEL and TRAP were used in combination of EMSA and IHC to determine SMMC7721/PMTr-neo proliferation and apoptosis.RESULTS Compared with the control, SMMC7721/PMTr-neo presented a significant drop in bcl-2 expression,accompanied by remarkable apoptosis and telomerase activity decline.CONCLUSION Bcl-2 ribozyme promotes apoptosis ofSMMC7721 cells and reduces telomerase activity. Thisprovides a theoretical basis for applying antisense technique to treatment of hepatecarcinoma. |
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| Keywords: | liver neoplasms Bcl-2 ribozyme apoptosis SMMC 7721 |
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