结肠癌p16基因缺失与突变的研究

目的 :探讨p16抑癌基因外显子 2缺失、突变与结肠癌发生、发展的关系。方法 :采用聚合酶链反应 (PCR)、聚合酶链反应—单链构象多态性 (PCR SSCP)分析方法检测结肠癌中p16基因外显子 2的缺失、突变。结果 :30例结肠癌样本中 ,高分化及低分化腺癌各 1例PCR扩增无扩增产物 ,可能为p16基因缺失 ;其余 2 8例结肠癌、正常组织均有产物出现。SSCP分析 ,30例结肠癌中未见异常泳动带 ,无p16基因突变。结论 :p16基因外显子 2缺失可能很少参与结肠癌的发生发展 ;而p16基因突变可能与结肠癌发生无关。

Deletion and Mutation of p16 Gene in Colorectal Carcinoma

Objective To evaluate the role of deletion and mutation of p16 gene in exon 2 in colorectal carcinoma.Methods The deletion and mutation of the p16 gene were respectively examined by polymerase chain reaction(PCR)and polymerase chain reaction single strand conformation polymorphism analysis(PCR SSCP)in colorectal carcinoma.Results The deletion of the p16 gene in exon 2 has been identified in 2 of 30 cases of primary colorectal carcinoma by PCR analysis.The mutation of the p16 gene in exon 2 has not been found in 30 cases of primary colorectal carcinoma by PCR SSCP analysis.Conclusions The deletion of the p16 gene in exon 2 may be involved in colorectal carcinogenesis.The mutation of the p16 gene in exon 2 may not be involved in colorectal carcinogenesis.