| 大鼠趋化素样因子2 mRNA在心肌梗死大鼠心脏中的表达 |
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| 引用本文： | 龚艳君,洪涛,蒋捷,于雪,卜定方,霍勇,高炜. 大鼠趋化素样因子2 mRNA在心肌梗死大鼠心脏中的表达[J]. 北京大学学报(医学版), 2003, 35(4): 438-440 |
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| 作者姓名： | 龚艳君 洪涛 蒋捷 于雪 卜定方 霍勇 高炜 |
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| 作者单位： | 1. 北京大学第一医院心内科,北京,100034
2. 北京大学第一医院中心实验室,北京,100034 |
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| 基金项目： | 教育部面向21世纪教育振兴行动计划(985计划);; |
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| 摘 要： | 目的：观察大鼠趋化素样因子2(rCKLF2)的mRNA在心肌梗死后大鼠心脏组织的表达。方法：构建大鼠心肌梗死模型，分别于心肌梗死后1d、3d、1周、2周、3周(每个时间点各5例)，取心肌梗死周边区心肌组织，提取组织总RNA，逆转录后行竞争性PCR，了解rCKLF2 mRNA的表达情况。结果：rCKLF2 mRNA在梗死心肌周边区的表达于术后上调，并呈动态变化，1周达峰值，为正常的6．7倍。结论：CKIF2可能参与心肌梗死的病理生理过程。
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| 关 键 词： | 大鼠 趋化素样因子 2mRNA 心肌梗死 心脏 表达 聚合酶链反应 |
| 文章编号： | 1671-167X(2003)04-0438-03 |
Expression of chemokine-like factor 2 (CKLF2) mRNA in the rat myocardium after myocardial infarction |
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| Yanjun Gong,Tao Hong,Jie Jiang,Xue Yu,Dingfang Bu,Yong Huo,Wei Gao. Expression of chemokine-like factor 2 (CKLF2) mRNA in the rat myocardium after myocardial infarction[J]. Journal of Peking University. Health sciences, 2003, 35(4): 438-440 |
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| Authors: | Yanjun Gong Tao Hong Jie Jiang Xue Yu Dingfang Bu Yong Huo Wei Gao |
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| Affiliation: | Department of Cardiology, Peking University First Hospital, Beijing 100034, China. |
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| Abstract: | Objective: To investigate the expression of chemokine like factor 2 (CKLF2) mRNA in the rat myocardium after myocardial infarction(MI).Methods: In a rat model of MI, the myocardium surrounding the infarcted area was used for RNA preparation at different time points. After RT, competitive polymerase chain reaction amplification was performed to assess the expression of rCKLF2 mRNA. SAS Kruskal Wallis test was used for statistical comparisons. Results: The gene expression of rCKLF2 at mRNA level was significantly increased in the myocardium surrounding the infarcted area 3 days, 1 week, 2 weeks after infarction.Conclusion: It is possible that CKLF2 contributes to the pathophysiological process and needs to be further investigated. |
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| Keywords: | Myocardial infarction Chemotactic factors Polymerase chain reaction |
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