人参皂甙对睡眠剥夺大鼠记忆保持的影响

目的：探讨人参皂甙（Ginsenosides,GS)对睡眠剥夺（sleep deprivation,SD)大鼠记忆保持的影响。方法：用小平台水环境法（Flower Pot)建立大鼠SD模型，选用Sprague-Dawley大鼠24只，根据SD时间的不同，将大鼠随机分为三组：24SD（24小时SD组），48SD（48小时SD组）和72SD（72小时SD组），每组又设实验组和对照组。其中实验组用GS连续灌胃5天，对照组以同样方式给予等量生理盐水，然后给予不同时间的SD。观察各组SD前后在跳台实验中的行为变化。结果：SD后各组大鼠跳台测试第一次触电潜伏期均下降，触电次数和触电时间均增加。SD前实验组和对照组的第一次触电潜伏期，触电次数和触电时间无显著差别（P＞0．05），72SD大鼠的第一次触电潜伏期实验组显著大于对照组（P＜0．05），触电次数和触电时间，实验组显著低于对照组（P＜0．05）；24SD，48SD的第一次触电潜伏期，触电次数和触电时间，实验组和对照组无显著差别。结论：连续口服GS对SD造成的大鼠记忆保持能力受损有明显保护作用，在一定睡眠剥夺时间内，这种保护作用随SD时间的延长而增强。

Effect of Ginsenosides onMemory Retention of Rats in Sleep Deprivation

Objective： To explore the effect of ginsenosides (GS) on the memory retention of rats in sleep deprivation (SD). Methods： SD was induced in male Sprague-Dawlay rats by employing “Flower Pot”technique. According to the time difference of being deprived of sleep, 24 rats were divided into three groups randomly: 24 SD (being deprived of sleep for 24 hours), 48 SD (being deprived of sleep for 48 hours), 72 SD (being deprived of sleep for 72 hours). And every group was also divided into two subgroups: experimental group and control group. Ginsenosides were forced fed to the experimental groups continuously for 5 days, and normal saline in equal volume to the controls, then the rats were being deprived of sleep for different time. Before and after SD immediately, the behaviors of rats were studied in Step-down test. Results ： Among all groups, the latency of first electrification decreased, and the number and time of electrification increased after SD. Compared with control groups, the latency of first electrification of experimental group increased significantly in 72 SD (p＜0.05), and the number and time of electrification decreased markedly (p＜0.05); there were no obvious difference in 24 SD and 48 SD (p＞0.05). And no difference was observed between experimental groups and the controls before SD (p＞0.05). Conclusion： GS showed a memory retention facilitating effect on rats with impaired memory due to SD in Step-down test, and the effect may be enhanced with increased duration of SD to a certain extent.