肾络通对5/6肾切除肾衰竭大鼠残余肾组织PAI-1蛋白表达的影响

目的观察肾络通对5/6肾切除肾衰竭大鼠残余肾组织纤溶酶原激活物抑制物-1(PAI-1)蛋白表达的影响。方法5/6肾切除制备肾衰竭大鼠模型,随机分为假手术组、模型组、肾络通组和缬沙坦组,分别灌胃给药16周取材,应用蛋白质印迹法检测肾组织PAI-1蛋白的表达。结果模型组与假手术组相比,PAI-1蛋白的表达明显增高(P<0.01);肾络通组PAI-1蛋白表达较模型组显著降低(P<0.05),与缬沙坦组比较无明显差异。结论肾络通可下调慢性进展性纤维化肾组织PAI-1蛋白表达,改善纤溶系统的失衡,延缓5/6肾切除肾衰大鼠的病程进展。

Influence of Shenluotong on expression of PAI-1 protein in kidney remaining tissue in rats with kidney failure induced by 5/6 of nephrectomy

Objective To observe the influence of Shenluotong on the protein expression of plasminogen activator inhibitor 1(PAI-1) in kidney remaining tissue in rats with kidney failure induced by 5/6 of nephrectomy.Mehtod The rat model of kidney failure was established by 5/6 of nephrectomy and the sham-operation group,model group,Shenluotong group and Valsartan group were set up and treated orally respectively for 16 weeks.The expression of PAI-1 protein in kidney tissue were detected by Western blotting method in different groups.Result The expression of PAI-1 protein was increased significantly in the model group compared with that in the sham-operation group(P<0.01),and that in the Shenluotong group was decreased significantly(P<0.05) without significant difference compared with that of the Valsartan group.Conclusion Shenluotong can down-regulate the expression of PAI-1 protein in kidney tissue of chronic developing fibrosis,relieve the unbalance of fibrinolysic system,and delay the development of rat kidney failure after 5/6 of nephrectomy.