缺血预处理对大鼠脑缺血再灌注后ICAM-1表达的影响

目的 通过研究脑缺血预处理对大鼠脑缺血再灌注后细胞间粘附分子-1(Intowellular adhesion molecule-1，ICAM-1)表达及多形核白细胞(Ploymorphonuclear leukocytes，PMNLs)浸润变化的影响．探讨预处理的延迟保护作用机制。方法采用四血管阻断法建立大鼠全脑-全脑缺血预处理模型。应用HE染色和免疫组化染色技术，观察脑缺血预处理后ICAM-1表达及多形核白细胞浸润的变化。结果(1)脑缺血再灌注后ICAM-1表达的阳性血管数量、PMNLs浸润数量均明显增加；(2)脑缺血预处理可明显减少缺血再灌注后ICAM-1的表达阳性血管数及PMNLs的浸润数量。结论一次性缺血预处理3min后48h可以少再次长时间缺血(30min)／再灌注(24h)的ICAM-1的表达和多形核白细胞浸润。ICAM-表达的下调和PMNLs浸润减少参与缺血预处理的保护作用。

The effect of ischemic preconditioning on the expression of intercellular adhesion molecule-1 induced by cerebral ischemia-reperfusion in rats

Objective To observe the effect of ischemic preconditioning on the expression of ICAM-1 induced by cerebral ischemia/reperfusion and to discuss the neuroprotective mechanism of ischemic preconditioning.Methods Using the model of 4-vessel occlusion and the technology of HE staining and immunohistochemical staining,we observed ICAM-1 expression and PMNLs infiltration.Results The expression of ICAM-1 and the quantity of PMNLs infiltration increased significantly after cerebral ischemia/reperfusion.Cerebral ischemic preconditioning inhibited the increase of neutrophils infiltration and ICAM-1 expression that induced by cerebral ischemia/reperfusion.Conclusions The results demonstrate that downregulate of ICAM-1 expression and the reduction of PMNLs infiltration are concerned with ischemic preconditioning neuroprotection.