| 131碘标记双功能单克隆抗体的制备鉴定与纯化及质量控制 |
| |
| 引用本文： | 杨家和,施乐华,钱光相,鄂亚军,吴孟超,殷广福. 131碘标记双功能单克隆抗体的制备鉴定与纯化及质量控制[J]. 临床肿瘤学杂志, 1997, 2(3): 11-13 |
| |
| 作者姓名： | 杨家和 施乐华 钱光相 鄂亚军 吴孟超 殷广福 |
| |
| 作者单位： | 第二军医大学东方肝胆医院 上海200433(杨家和，施乐华，钱光相，郭亚军，吴孟超)，解放军第81医院全军肿瘤中心 南京210002(殷广福) |
| |
| 摘 要： | 目的:本实验在于研究BiMcAB介导免疫治疗的可能性,因双抗是生物制剂,有必要进行质量控制。方法:采用硫酸胺盐析法纯化蛋白,及氯胺法标记蛋白,并进行灭菌检查。结果:BiMcAB的理化特性,生物学鉴定,放射化学指标,生物及免疫学指标均符合临床应用标准。结论:BiMcAB有可能成为一种既能定向杀伤癌细胞,而对正常细胞又无损害作用的安全的新的治疗途径。
|
| 关 键 词： | 双功能抗体 ~(131)碘标记 质量控制 |
Preparation appraisal purification and quality control for 131 _(I)-labelled bispecific monoclonal antibody |
| |
| Yang Jiahe,Shi Lehua,Yin Guangfu,et al.. Preparation appraisal purification and quality control for 131 _(I)-labelled bispecific monoclonal antibody[J]. Chinese Clinical Oncology, 1997, 2(3): 11-13 |
| |
| Authors: | Yang Jiahe Shi Lehua Yin Guangfu et al. |
| |
| Affiliation: | Yang Jiahe,Shi Lehua,Yin Guangfu,et al. Shanghai East Hospital of Hepatobiliary Surgery,Shanghai 200433 |
| |
| Abstract: | Purpose: In order to realize the practicability 0f immunoimagmg and treatment with bispecific monoclonal anti-body, a series of experiment was undertaken in our institute. Methods: The pure antibody was obtained by salt out method Protein-G method, and chioramine-T method was used for label. Sterility and pyrogen tests were carried out before administra-tion. Results: All results above were coincident with quality for clinical application. Conclusion: The bispeific monoclonal anti-body does not damage normal cells,but kill the carcinoma cells selectively,and may be a new therapy method. |
| |
| Keywords: | Bispecific monoclonal 131I label Quality Contral |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
