Low‐level laser therapy in collagenase‐induced Achilles tendinitis in rats: Analyses of biochemical and biomechanical aspects |
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| Authors: | Rodrigo Labat Marcos Ernesto Cesar Pinto Leal‐Junior Gilles Arnold Vincent Magnenet Rachid Rahouadj Xiong Wang Frank Demeurie Jacques Magdalou Maria Helena Catelli de Carvalho Rodrigo Álvaro Brandão Lopes‐Martins |
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| Institution: | 1. Laboratory of Pharmacology and Experimental Therapeutics, Department of Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo (USP), Av. Prof. Lineu Prestes, 1524, Butantan, S?o Paulo 05508‐900, SP, Brazil;2. Laboratoire de Physiopathologie, Pharmacologie et Ingénierie Articulaires (LPPIA), UMR 7561 CNRS, Nancy‐Université, Nancy, France;3. Post Graduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University (UNINOVE), S?o Paulo, SP, Brazil;4. Post Graduate Program in Rehabilitation Sciences, Nove de Julho University (UNINOVE), S?o Paulo, SP, Brazil;5. Université de Haute Alsace (UHA), Laboratoire Physique et Mécanique Textile (LPMT), EA 4365, F‐68093 Mulhouse, France;6. Laboratoire d'Energétique et de Mécanique Théorique et Appliquée (LEMTA), UMR 7563 du CNRS, 2, Avenue de le Forêt de Hayes, 54500 Vand?uvre‐lès‐Nancy, Nancy‐Université, Nancy, France;7. LAEGO‐ENSG, Rue du Doyen Marcel Roubault, B.P. 40, 54500 Vand?uvre‐lès‐Nancy, Nancy‐Université, Nancy, France;8. Laboratory of Hypertension, Department of Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo (USP), S?o Paulo, SP, Brazil |
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| Abstract: | NSAIDs are widely prescribed and used over the years to treat tendon injuries despite its well‐known long‐term side effects. In the last years several animal and human trials have shown that low‐level laser therapy (LLLT) presents modulatory effects on inflammatory markers, however the mechanisms involved are not fully understood. The aim of this study was to evaluate the short‐term effects of LLLT or sodium diclofenac treatments on biochemical markers and biomechanical properties of inflamed Achilles tendons. Wistar rats Achilles tendons (n = 6/group) were injected with saline (control) or collagenase at peritendinous area of Achilles tendons. After 1 h animals were treated with two different doses of LLLT (810 nm, 1 and 3 J) at the sites of the injections, or with intramuscular sodium diclofenac. Regarding biochemical analyses, LLLT significantly decreased (p < 0.05) COX‐2, TNF‐α, MMP‐3, MMP‐9, and MMP‐13 gene expression, as well as prostaglandin E2 (PGE2) production when compared to collagenase group. Interestingly, diclofenac treatment only decreased PGE2 levels. Biomechanical properties were preserved in the laser‐treated groups when compared to collagenase and diclofenac groups. We conclude that LLLT was able to reduce tendon inflammation and to preserve tendon resistance and elasticity. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:1945–1951, 2012 |
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| Keywords: | anti‐inflammatory drugs LLLT phototherapy tendon biomechanics |
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