Biosynthesis of glucose‐d‐13C6 from Hansenula polymorpha and methanol‐13C

A simple and effective method for synthesis of glucose‐d ‐¹³C₆ by fermentation using the methylotrophic yeast Hansenula polymorpha with 99% abundance methanol‐¹³C is described. Using methanol‐¹³C as a sole source of carbon, H. polymorpha can accumulate large amounts of α,α‐trehalose‐¹³C₁₂ under unfavourable growth conditions; the trehalose can then be hydrolysed to give glucose‐d ‐¹³C₆ with 98.5% abundance ¹³C. Copyright © 2011 John Wiley & Sons, Ltd.