大鼠胚胎卵巢异体异位移植的探讨

为了研究胚胎卵巢移植后的存活和生长发育情况 ,以及不同移植部位对胚胎卵巢的影响 ,将大鼠胚胎卵巢分别移入去势雌鼠的颈部皮下及肾被膜下 ,HE观察颈部移植物移植后 5d处于坏死状态 ,含有大量红细胞 ;1 0d周围有结缔组织包裹 ,内有原始卵泡 ;2 5d及以后移植物内存在大量发育卵泡并可见间质腺及黄体。肾被膜移植物移植后 5d呈结缔组织样 ;1 0d出现大量原始卵泡结构 ;2 5d及以后可见发育卵泡、间质腺和黄体。细胞化学显示发育卵泡的 3 β羟甾脱氢酶呈阳性反应。移植后 2 5d左右阴道涂片出现动情周期。结果说明胚胎卵巢能在异体异位存活并可发育成具有内分泌功能的腺体。

A Study of Allotransplanted Fetal Ovary in Rat

Seventeen day old to nineteen day old fetal ovaries was allotransplanted heterotopically(under the renal capsule and subcutaneous tissure in neck) to bilaterally ovariectomized adult Wistar rat.Restoration of cyclic activity about 25 day or so after transplantation was found by observing ovariectomized adult recipient vaginal smears.Transplanted ovarian necrosis can be observed on day 5 after transplantation in subcutaneous group,there was no obvious necrosis in renal capsule group. Both of the two groups have a lot of primordial follicles on the day 10. Developing follicles of different stages,corpora lutea and interstitial gland existed on day 25 or later. This study showed that the allotransplanted fetal ovaries could live,grow well and secret steroid hormone.