Comparative Study of Cytokine Content in the Plasma and Wound Exudate from Children with Severe Burns |
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Authors: | E V Mikhal’chik J A Piterskaya L Y Budkevich L Yu Pen’kov A Facchiano C De Luca G A Ibragimova and L G Korkina |
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Institution: | 1.Russian State Medical University,Moscow,Russia;2.Italian Dermatology Institute,Rome,Italy;3.G. N. Speranskii Children’s Municipal Clinical Hospital No. 9,Moscow,Russia |
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Abstract: | The content of 27 cytokines was measured in blood plasma from 19 children with severe uncomplicated burns (group 1) and complicated
burns (septic toxemia, toxemia, and pneumonia; group 2). Before surgical treatment (day 4 (±2) after burn), significant differences
were found in the concentrations of interleukin-1 receptor antagonist, interleukin-6, interleukin-8, interleukin-10, tumor
necrosis factor-α, interferon-γ, MCP-1, and granulocyte colony-stimulating factor. Cytokine concentration in group 2 patients
was much higher than in group 1 patients and healthy children. The concentrations of interleukin-6, interleukin-8, and MCP-1
in group 1 patients significantly surpassed the normal level. Cytokine concentration in the plasma and wound exudates and
myeloperoxidase activity in wound exudates from 4 patients of group 2 were measured over 18 days after burn. The inflammatory
response was characterized by an increase in the content of interleukin-1β, interleukin-8, MCP-1, tumor necrosis factor-α,
MIP-1α, and granulocyte-macrophage colony-stimulating factor in the wound (as compared to that in the plasma). Activity of
myeloperoxidase in all patients was shown to correlate with the amount of MIP-1α (r=0.47), tumor necrosis factor-α (r=0.47), and granulocyte-macrophage colony-stimulating factor (r=0.55, p<0.05). Interleukin-8 concentration was beyond the limits of calibration. No correlation was found between the concentration
of any of 27 cytokines in blood plasma and exudate. Our results indicate that during active surgical treatment, the wound
serves as the source of inflammatory cytokines. Cytokines play a role in the systemic response and increase the degree of
local inflammation, which modulates the number and activity of wound neutrophils. |
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