GC法测定消炎止痒醑中薄荷脑的含量

目的建立GC法测定消炎止痒醑中薄荷脑的含量。方法采用CP-Sil 5 CB色谱柱(25m×0.32mm×0.4μm),FID检测器。进样口温度250℃;柱温280℃(程序升温:初始温度60℃,保持1min,再以12℃/min的速度升至280℃,保持3min);检测器温度300℃;分流进样,分流比20:1;进样量1μL。结果薄荷脑进样量在50.8～609.6mg/L范围内与峰面积呈良好线性关系,r=0.999 69,平均回收率为99.10%,RSD为0.87%(n=6)。结论本测定方法简便、快速、准确、重复性好,可作为消炎止痒醑的含量测定方法。

Determination of Menthol in Anti-inflammatory Antipruritic Spiritus by GC

Objective To establish a method for the determination of menthol in anti-inflammatory antipruritic spiritus by GC.Methods GC was performed on a CP-Sil 5 CB column(25m × 0.32mm × 0.4μm) using FID as the detector.Inlet temperature was set at 250 ℃ and column temperature was 280 ℃(temperature program: initial temperature 60 ℃,maintained 1min,then rise to 280 ℃ with the rate of 12 ℃ / min,maintained 3min);detector temperature was 300 ℃;split injection,split ratio 20:1;injection volume 1μL.Results The standard curve was linear in the range of 50.8 ~ 609.6mg / L,r = 0.999 69,the average recovery was 99.10% with RSD of 0.87%(n = 6).Conclusion The method was simple,fast,accurate,reproducible and could be used for the determination of menthol in anti-inflammatory antipruritic spiritus.