牛磺酸对高温处理后神经上皮细胞存活和分化的影响

目的 探讨牛磺酸对高温处理后神经上皮细胞存活和分化的作用。 方法 取孕12.5d小鼠胚胎神经管上皮细胞进行原代培养,将其分为正常对照组、高温组和牛磺酸保护Ⅰ、Ⅱ组。显微镜下观察细胞的形态学变化,用四甲基偶氮唑盐(MTT)自动比色法测定细胞增殖活性,二脒基苯基吲哚(DAPI)染色法测定细胞凋亡率,免疫荧光法检测细胞中Caspase-3、微管相关蛋白-2(MAP-2)和胶质纤维酸性蛋白(GFAP)的表达。 结果 与正常对照组比较,高温组细胞增殖活性明显降低,细胞凋亡率明显升高,Caspase-3阳性细胞数明显增多,MAP-2阳性细胞数明显减少;与高温组比较,牛磺酸保护Ⅰ、Ⅱ组细胞活性显著升高,凋亡率显著降低,Caspase-3阳性细胞数明显减少,MAP-2表达明显增多;4个组比较,GFAP的表达差异无显著性。 结论 牛磺酸对高温处理后神经管上皮细胞的存活具有保护作用,并可能促进其向神经元方向分化。

Effects of taurine on the survival and differentiation of neuroepithelial cells treated with hyperthermia

Objective To study the effects of taurine on the survival and differentiation of neuroepithelial cells treated with hyperthermia. Methods Neuroepithelial cells were dissociated from embryonic neural tube of E12.5d mice. They were divided into the control group, the hyperthermia group and the taurine groupⅠ,Ⅱ. The morphological changes were observed under the light microscope. MTT was used to examine the proliferative activitiy of various groups. Apopotosis percentage was determined by DAPI staining. The immunofluorescence staining was used to identify the expression of the Caspase-3, MAP-2 and GFAP in cultured cells. The immunostaining positive cells were counted at high fields in different groups. Results In the hyperthermia group, compared to the control group,the proliferative activitiy of neuroepithelial cells decreased significantly, the apoptosis percentage and the immunostaining positive cells of Caspase-3 increased, the immunostaining positive cells of MAP-2 decreased. However, these changes were significantly reversed by the taurine pre conditioning. Conclusion Taurine may protect neuroepithelial cells from hyperthermia, and may promote these cells to differentiate i