| 人正常肾上腺及APA组织培养及其在心钠素作用下醛固铜量... |
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| 引用本文： | 王瑞瑜,孙梅励.人正常肾上腺及APA组织培养及其在心钠素作用下醛固铜量...[J].中国医学科学院学报,1991,13(3):181-184. |
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| 作者姓名： | 王瑞瑜 孙梅励 |
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| 摘 要： |
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| 关 键 词： | 组织培养 心钠素 醛固酮 |
Establishment of human adrenal gland and aldosterone-producing adenoma culture and the effect of alpha-human atrial natriuretic factor on aldosterone secretion in vitro] |
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| R Wang.Establishment of human adrenal gland and aldosterone-producing adenoma culture and the effect of alpha-human atrial natriuretic factor on aldosterone secretion in vitro][J].Acta Academiae Medicinae Sinicae,1991,13(3):181-184. |
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| Authors: | R Wang |
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| Institution: | Institute of Basic Medical Sciences, Beijing. |
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| Abstract: | Human adrenal gland and aldosterone-producing adenoma (APA) obtained from adrenalectomy could be maintained in vitro for one to two months. The explants continued to secrete aldosterone for the duration of culture. This method was superior to monolayer cell culture. The influence of alpha-human atrial natriuretic factor (alpha-hANF) was investigated by treating cultures of normal adrenal cortex and APA with alpha-hANF (10(-8) mol/L and 3 x 10(-8) mol/L respectively). We measured aldosterone in culture media collected at 2h before alpha-hANF treatment and 0.5, 2, 4 and 24h after. It was found that alpha-hANF stimulated aldosterone secretion of normal adrenal tissue but slightly inhibited that of APA. |
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