| 分离纯化肾小管基底膜的改进方法 |
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| 引用本文： | 万虹,许以侣,张家颖,赵风章. 分离纯化肾小管基底膜的改进方法[J]. 吉林大学学报(医学版), 1988, 0(4) |
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| 作者姓名： | 万虹 许以侣 张家颖 赵风章 |
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| 作者单位： | 白求恩医科大学组织胚胎教研室(万虹，许以侣)，白求恩医科大学中心实验室(张家颖)，白求恩医科大学中心实验室(赵风章) |
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| 摘 要： | 本实验采用分样筛、磁性分离和密度梯度离心综合法分离猪肾皮质的肾小管基底膜。相差显微镜观察肾小管的纯度可达97％以上。磁性分离后用超声震碎细胞核,再经密度梯度离心,即可获得高纯度的肾小管基底膜。
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| 关 键 词： | 肾小管基底膜 超声震碎 分样筛 磁性分离 密度梯度离心 区带 |
Improoved method of isolation and purification of tubular basement membrane |
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| Wan Hong,Xu Yilu,Zhang Jiaying and Zhao Fengzhang. Improoved method of isolation and purification of tubular basement membrane[J]. Journal of Jilin University: Med Ed, 1988, 0(4) |
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| Authors: | Wan Hong Xu Yilu Zhang Jiaying Zhao Fengzhang |
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| Abstract: | A combined method of graded sieving, magnetic separating and density gradient centrifugating procedure wae used to isolate tubular basement membrane from renal cortex of pigs. The degree of purity of the tubules thus prepared was above 97% by using phase microscopy. After magnetic separation of the tubules,the nuclei were disrupted in a ultrasonicator,and finally the highly puri-fied tubular basement was obtained by discontinuous sucrose density gradient centrifugation. |
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| Keywords: | Tubular basement membrane Ultrasonic disruption Sieving Magnetic separation Den sity gradient centrifugation Band |
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