康复新栓对慢性阴道炎大鼠血清MDA、SOD及组织PGE2的影响

目的探讨康复新栓对慢性阴道炎大鼠血清中丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)及阴道组织中前列腺素E2(prostaglandin E2,PGE2)含量的影响。方法以大肠埃希菌、白色假丝酵母菌、金黄色葡萄球菌混合菌液和8%苯酚胶液交叉感染建立大鼠阴道炎模型。经阴道给予空白栓,双唑泰栓(37.84 mg·kg^-1),高剂量(80mg·kg^-1)、低剂量(40mg·kg^-1)康复新栓治疗。通过酶联免疫法检测大鼠血清中MDA、SOD和阴道组织PGE2含量,通过病理组织学评分判断其阴道炎程度。结果与正常对照组比较,模型对照组大鼠血清中MDA和阴道组织匀浆中PGE2含量显著升高(P<0.01),SOD活力显著降低(P<0.01);大鼠阴道病理组织学评分显著增高(P<0.01),说明大鼠阴道炎模型造模成功。与模型对照组比较,双唑泰栓组、康复新栓高、低剂量组血清SOD活性显著升高(P<0.01),MDA及PGE2含量显著降低(P<0.01);大鼠阴道病理组织学评分显著降低(P<0.01)。结论康复新栓能有效治疗大鼠的慢性阴道炎,可能与降低大鼠血清中MDA的表达及提高阴道组织中SOD的活性,改善阴道组织病变程度有关。

Effect of Kangfuxin Suppository on Serum MDA, SOD and Tissue PGE2 in Rats with Chronic Vaginitis

OBJECTIVE To investigate the effect of Kangfuxin suppository on the contents of malondialdehyde(MDA) and superoxide dismutase(SOD) in serum and prostaglandin E2(PGE2) in vaginal tissue of rats with chronic vaginitis. METHODS The rat vaginitis model was established by cross infection of Escherichia coli, Candida albicans, Staphylococcus aureus mixture and 8% phenolic gel. Transvaginal administration of blank suppository, metronidazole suppository (37.84 mg·kg^-1), high dose (80 mg·kg^-1) and low dose (40 mg·kg^-1) of Kangfuxin suppository. The contents of MDA, SOD in serum and PGE2 in vaginal tissue of rats were detected by ELISA. The degree of vaginitis was diagnosed by histopathological score. RESULTS Compared with normal control group, the level of MDA in serum and PGE2 content in vaginal homogenate of rats in model control group increased significantly(P<0.01), SOD activity decreased significantly(P<0.01). The histopathological score of rats'' vagina was significantly increased(P<0.01), indicating that the rat vaginitis model was successfully established. Compared with the model control group, the serum SOD activity in the metronidazole suppository group, Kangfuxin suppository high and low groups increased significantly(P<0.01), and the MDA and PGE2 contents decreased significantly(P< 0.01). The histopathological score of rats'' vagina was significantly decreased(P<0.01). CONCLUSION Kangfuxin suppository can effectively treat chronic vaginitis of rats, which may be related to reducing the expression of MDA in rats'' serum, increasing the activity of SOD in vaginal tissue and changing the degree of pathological changes in vaginal tissue.