金丝梅石油醚超声提取物GC-MS分析及其体外抗氧化作用

目的采用GC-MS分析金丝梅(HypericumpatalumThumb.)石油醚超声提取物中的化学成分,并研究该提取物的抗氧化作用。方法以石油醚为溶剂,采用超声提取法提取金丝梅中的化学成分,并采用GC-MS分析石油醚超声提取物中的化学成分;采用DPPH和ABTS2种方法研究金丝梅石油醚超声提取物的抗氧化活性。结果从金丝梅石油醚超声提取物中共鉴定了24个化合物。其中,3-甲基庚烷含量最高,达26.329%,检测出的化合物类型为烷烃类(57.99%)、萜类(17.22%)、芳烃(7.78%)和其他类型(17.01%)化合物。该提取物对DPPH自由基和ABTS^·+自由基的清除作用在一定浓度范围内成对数关系,对DPPH自由基清除作用的IC50值>20mg·mL^-1,对ABTS^·+自由基的清除作用TEAC值为(3.71±0.1)μmol·L^-1。结论金丝梅石油醚超声提取物中脂溶性成分较多,具有一定的抗氧化活性。该研究结果为金丝梅化学成分的研究及进一步利用提供了科学依据。

Compositional Analyses by GC-MS and Antioxidant Activities in Vitro of Petroleum Ether Extract of Hypericum Patulum Thumb. Under Ultrasound Irradiation

OBJECTIVE To study the chemical constituents by GC-MS and antioxidant activities of petroleum ether extract of Hypericum patulum Thumb.under ultrasound irradiation.METHODS The chemical constituents of Hypericum patulum Thumb.were extracted with petroleum ether using the ultrasonic extraction method and analyzed by GC-MS.Furthermore,the antioxidant activities of petroleum ether extract were measured by DPPH and ABTS·+radicals scavenging activity assays.RESULTS The 24 compounds were identified from the petroleum ether extract of Hypericum patulum Thumb.under ultrasound irradiation,3-methyl heptane with relative content of 26.329%was the most among them.The types of detected compounds were alkane(57.99%),terpenoids(17.22%),aromatics(7.78%)and others(17.01%).The DPPH radical scavenging effect and ABTS^·+radical scavenging effect were individually in logarithm relation with concentration in a certain range.The IC50 value of DPPH radical scavenging was>20 mg·mL^-1,the TEAC value of ABTS^·+radical scavenging effect was(3.71±0.1)μmol·L^-1.CONCLUSION The liposoluble constituents of Hypericum patulum Thumb.are abundant,and show certain antioxidant activity,which offer a scientific basis for further chemical study and utilization of Hypericum patulum Thumb..