银杏叶渣中多糖的提取及其抗氧化活性研究

目的 研究从银杏叶渣中提取多糖的工艺,及其抗氧化活性。方法 以多糖提取率为评价指标,通过苯酚-硫酸法检测,经单因素试验得到最佳工艺,所得银杏多糖进行还原能力和清除DPPH自由基的试验。结果 最佳工艺条件为：料液比1：7,提取时间2h,提取2次,醇沉浓度90%,醇沉1h。银杏多糖具有较强的还原能力和清除DPPH自由基的能力。结论 该工艺简便可行,稳定可靠,适于工业生产,所得多糖具有较强的抗氧化活性。

Extraction of polysaccharides from residue of Ginkgo Biloba and study on anti-oxidant activity

Objective: To develop the optimal extraction technology of polysacchrides from residue of Ginkgo Biloba , and conduct anti-oxidant tests. Methods: Taking the extraction ratio of polysaccharides for evaluation index and detected by phenol-sulfuric acid method, the optimal technology is obtained through single factor experiments. And experiments are taken on reducing activity and scavenging activity against DPPH. Results :The optimal extraction conditions are material-to-liquid ratio of 1:7, extraction time of 2 hours, 2 times, alcohol precipitation concentration of 90%, alcohol precipitation time of 1 hours . The polysaccharides obtained have a rather good reducing activity and scavenging activity against DPPH . Conclusion :The technology is convenient and workable, stable and dependable, which is suitable for industrial manufacture. The polysaccharides obtained have strong anti-oxidant activities.